Assessment of umbilical cord tissue as a source of mesenchymal stem cell/endothelial cell mixtures for bone regeneration

Dimitrios Kouroupis, Sarah M. Churchman, Anne English, Paul Emery, Peter V. Giannoudis, Dennis Mcgonagle, Elena A. Jones

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Aim: To enumerate and characterize mesenchymal stem cells (MSCs) and endothelial cells (ECs) in umbilical cord (UC) tissue digests. Materials & methods: Cultured UC cells were characterized phenotypically, and functionally by using 48-gene arrays. Native MSCs and ECs were enumerated using flow cytometry. Results: Compared with bone marrow (BM) MSCs, UC MSCs displayed significantly lower (range 4-240-fold) basal levels of bone-related transcripts, but their phenotypes were similar (CD73+, CD105+, CD90+, CD45- and CD31-). UC MSCs responded well to osteogenic induction, but day 21 postinduction levels remained below those achieved by BM MSCs. The total yield of native UC MSCs (CD90+, CD45- and CD235α-) and ECs (CD31+, CD45- and CD235α-) exceeded 150 and 15 million cells/donation, respectively. Both UC MSCs and ECs expressed CD146. Conclusion: While BM MSCs are more predisposed to osteogenesis, UC tissue harbors large numbers of MSCs and ECs; such minimally manipulated 'off-the-shelf' cellular mixtures can be used for regenerating bone in patients with compromised vascular supply.

Original languageEnglish (US)
Pages (from-to)569-581
Number of pages13
JournalRegenerative Medicine
Volume8
Issue number5
DOIs
StatePublished - Sep 2013
Externally publishedYes

Keywords

  • bone marrow
  • bone regeneration
  • endothelial cells
  • mesenchymal stem cells
  • umbilical cord

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

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