Assessment of transport of lipid metabolites within trabecular meshwork cells

Daphne Toglia, Stephanie Dancausse, Karthik Kalahasty, Bryan Alfonso, Sanjoy K. Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lipids from trabecular meshwork (TM) cells are of particular interest to ophthalmological researchers as a therapeutic target for lowering intraocular pressure (IOP) in glaucomatous eyes. Fluorescence-based lipid transport assays (FBLTA) and immunocytochemistry (ICC) are dynamic fluorescence analysis techniques that allow for quantitative and qualitative comparisons, respectively, between multiple samples. Here we describe methods for FBLTA, ICC, and mass spectroscopy designed to measure the kinetics and localization of lipid metabolites within the trabecular meshwork.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages187-197
Number of pages11
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell counting
  • Cell culture
  • Fluorescence-based lipid transport assay
  • Immunocytochemistry
  • Lipid extraction
  • Lipidomics
  • Lipids
  • Mass spectrometry
  • Metabolites

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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