Assessment of transport of lipid metabolites within trabecular meshwork cells

Daphne Toglia, Stephanie Dancausse, Karthik Kalahasty, Bryan Alfonso, Sanjoy K Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lipids from trabecular meshwork (TM) cells are of particular interest to ophthalmological researchers as a therapeutic target for lowering intraocular pressure (IOP) in glaucomatous eyes. Fluorescence-based lipid transport assays (FBLTA) and immunocytochemistry (ICC) are dynamic fluorescence analysis techniques that allow for quantitative and qualitative comparisons, respectively, between multiple samples. Here we describe methods for FBLTA, ICC, and mass spectroscopy designed to measure the kinetics and localization of lipid metabolites within the trabecular meshwork.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages187-197
Number of pages11
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745

Fingerprint

Trabecular Meshwork
Lipids
Fluorescence
Immunohistochemistry
Intraocular Pressure
Mass Spectrometry
Research Personnel

Keywords

  • Cell counting
  • Cell culture
  • Fluorescence-based lipid transport assay
  • Immunocytochemistry
  • Lipid extraction
  • Lipidomics
  • Lipids
  • Mass spectrometry
  • Metabolites

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Toglia, D., Dancausse, S., Kalahasty, K., Alfonso, B., & Bhattacharya, S. K. (2019). Assessment of transport of lipid metabolites within trabecular meshwork cells. In Methods in Molecular Biology (pp. 187-197). (Methods in Molecular Biology; Vol. 1996). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9488-5_16

Assessment of transport of lipid metabolites within trabecular meshwork cells. / Toglia, Daphne; Dancausse, Stephanie; Kalahasty, Karthik; Alfonso, Bryan; Bhattacharya, Sanjoy K.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 187-197 (Methods in Molecular Biology; Vol. 1996).

Research output: Chapter in Book/Report/Conference proceedingChapter

Toglia, D, Dancausse, S, Kalahasty, K, Alfonso, B & Bhattacharya, SK 2019, Assessment of transport of lipid metabolites within trabecular meshwork cells. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1996, Humana Press Inc., pp. 187-197. https://doi.org/10.1007/978-1-4939-9488-5_16
Toglia D, Dancausse S, Kalahasty K, Alfonso B, Bhattacharya SK. Assessment of transport of lipid metabolites within trabecular meshwork cells. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 187-197. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9488-5_16
Toglia, Daphne ; Dancausse, Stephanie ; Kalahasty, Karthik ; Alfonso, Bryan ; Bhattacharya, Sanjoy K. / Assessment of transport of lipid metabolites within trabecular meshwork cells. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 187-197 (Methods in Molecular Biology).
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