Assessment of the malignant potential of mitogen stimulated human Schwann cells

Evelyne Emery, Xiuming Li, J. P. Brunschwig, Leslie Olson, Allan D Levi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Human Schwann cells (SCs) can be isolated and expanded with mitogens using cell culture techniques. These cells have been demonstrated to promote axonal regrowth in both the central and peripheral nervous system. Primary rat SCs can be immortalized with long-term exposure to mitogens. Transplantation of these cells into an autogenic host sciatic nerve results in the formation of tumors. Human cells are, in general, relatively more resistant to malignant transformation, but any potential risk for inducing tumor after transplantation of SCs in humans must be excluded. In this study, the malignant potential of mitogen expanded human SCs injected into the sciatic nerve of immunodeficient mice was investigated. Human SCs were isolated from human peripheral nerves and placed in cell culture, expanded with mitogens (heregulin and forskolin) for many passages (0-6 times), and then injected within the sciatic nerve of Severe Combined Immunodeficient (SCID) rat or mice. As a positive control for tumor formation in this xenograft model, human glioma cells were also injected. The proliferation index (PI) of the human SCs gradually decreased with each passage in cell culture. SC purity remained stable until the 6th passage, and then decreased significantly for older passages, so that the cultures were over-grown with fibroblasts. The incidence for rat or human glioma cells to induce tumors was 100% and 92%, respectively. In contrast, there was no tumor induced by human primary or mitogen expanded SCs. Demyelination, remyelination and formation of connective sheath at the injection site were observed in some cases after injection of the human SCs. Thus, mitogen-expanded human SCs do not produce tumors when transplanted in vivo, which suggests that these cells are safe, and deserve further study towards their use in clinical transplantation.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalJournal of the Peripheral Nervous System
Volume4
Issue number2
StatePublished - Aug 11 1999

Fingerprint

Schwann Cells
Mitogens
Sciatic Nerve
Neoplasms
Cell Culture Techniques
Glioma
Transplantation
Neuregulin-1
Injections
Cell Transplantation
Peripheral Nervous System
Demyelinating Diseases
Colforsin
Peripheral Nerves
Heterografts
Central Nervous System
Fibroblasts

Keywords

  • Cell line
  • Neoplasm
  • Schwann cell
  • SCID mice
  • Transplantation

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Assessment of the malignant potential of mitogen stimulated human Schwann cells. / Emery, Evelyne; Li, Xiuming; Brunschwig, J. P.; Olson, Leslie; Levi, Allan D.

In: Journal of the Peripheral Nervous System, Vol. 4, No. 2, 11.08.1999, p. 107-116.

Research output: Contribution to journalArticle

Emery, Evelyne ; Li, Xiuming ; Brunschwig, J. P. ; Olson, Leslie ; Levi, Allan D. / Assessment of the malignant potential of mitogen stimulated human Schwann cells. In: Journal of the Peripheral Nervous System. 1999 ; Vol. 4, No. 2. pp. 107-116.
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