Assessment of the expression of IRβ, IRS-1, IRS-2 and IGF-IRβ in a rat model of intrauterine growth restriction

Marcia Pereira Bueno, Dioze Guadagnini, Frances Lilian Lanhellas Gonçalves, Ricardo Barini, Mario José Abdalla Saad, Augusto Frederico Schmidt, Lourenço Sbragia

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Objective: To investigate glomerular development and expression of insulin and insulin-like growth factor receptors in an experimental model of intrauterine growth restriction (IUGR). Material and Methods: We studied three groups of Sprague-Dawley fetuses: IUGR - restricted by ligation of the right uterine artery; C-IUGR - left horn controls, and EC - external controls (non-manipulated). Body and organs were weighed, and glomerular number and volume were analyzed. Expression of IRβ, IRS-1, IRS-2 and IGF-IRβ was analyzed in liver, intestine and kidneys by immunoblotting. Results: Organ/body weight ratios were similar. In IUGR, glomerular number and volume were increased compared to C-IUGR and EC (p<0.001). In the IUGR liver, increases were found in IGF-IRβ compared to C-IUGR and EC; IRβ compared to EC, and IRS-2 compared to C-IUGR. However, decreases in IRβ were noted in IUGR compared to C-IUGR; IRS-1 compared to C-IUGR and EC, and IRS-2 compared to EC. In IUGR intestine, increases were detected in IRβ, IRS-1 and IGF-IRβ compared to C-IUGR and EC. In IUGR kidneys, increases were observed in IRβ and IGF-IRβ compared to C-IUGR and EC, and IRS-1 compared to EC. Decreased IRS-2 in the intestine and kidney were noticed in IUGR compared to C-IUGR and EC. Conclusion: IUGR fetuses had less glomeruli and alterations in insulin receptors, which may be associated with an increased risk of disease occurrence in adulthood.

Original languageEnglish (US)
Pages (from-to)145-152
Number of pages8
JournalFetal Diagnosis and Therapy
Volume28
Issue number3
DOIs
StatePublished - Oct 2010
Externally publishedYes

Keywords

  • Fetal glomerular development
  • Glomeruli
  • Insulin receptor
  • Insulin-like growth factor receptor
  • Intrauterine growth restriction

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Embryology
  • Radiology Nuclear Medicine and imaging
  • Obstetrics and Gynecology

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