Assessment of the effectiveness of BG-Sentinel traps baited with CO 2 and BG-Lure for the surveillance of vector mosquitoes in Miami-Dade County, Florida

André B.B. Wilke, Augusto Carvajal, Johana Medina, Melissa Anderson, Veronica J. Nieves, Monica Ramirez, Chalmers Vasquez, William Petrie, Gabriel Cardenas, John C Beier

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3 Scopus citations

Abstract

Vector-borne diseases are an increasing issue to public health, endangering billions of people worldwide. Controlling vector mosquitoes is widely accepted as the most effective way to prevent vector-borne disease outbreaks. Mosquito surveillance is critical for the development of control strategies under the integrated vector management framework. We hypothesize that the effectiveness and reliability of using BG-Sentinel traps for the surveillance strongly depend on the bait used to attract mosquitoes. The objective of this study was to compare the effectiveness of BG-Sentinel traps baited with CO 2 and BG-Lure. A total of 72 traps were deployed for 48 hours once a week for four weeks. For the initial 24-hour period, the traps were baited with CO 2 , and then for an additional 24 hours using the BG-Lure. Collected mosquitoes were analyzed using the Generalized Estimating Equation for repeated measures analysis. Biodiversity was assessed by the Shannon and Simpson indices and by individual rarefaction curves and SHE profiles. A total of 5,154 mosquitoes were collected, from which 3,514 by traps baited with CO 2 and 1,640 mosquitoes by traps baited with BG-Lure. Aedes aegypti and Culex quinquefasciatus were the most abundant and dominant species. Results from the Generalized Estimating Equation models indicated that more than twice as many mosquitoes were attracted CO 2 than to the BG-Lure. The comparison of attractiveness of CO 2 and BG-Lure to Ae. aegypti and Cx. quinquefasciatus was non-significant, suggesting that both species were equally attracted by the baits. The individual rarefaction curves for Ae. aegypti and Cx. quinquefasciatus imply that traps baited with BG-Lure underestimated mosquito species richness compared to those baited with CO 2 . BG-Lure were less effective in attracting mosquitoes with low abundances and failed to collect Cx. coronator and Cx. nigripalpus, which were consistently collected by traps baited with CO 2 . According to our results, CO 2 significantly (P<0.05) attracted more mosquitoes (2.67 adjusted odds ratios) than the BG-Lure when adjusted for time and species, being more effective in assessing the relative abundance of vector mosquitoes and yielding more trustworthy results. Traps baited with CO 2 collected not only more specimens, but also more species in a more consistent pattern.

Original languageEnglish (US)
Article numbere0212688
JournalPloS one
Volume14
Issue number2
DOIs
StatePublished - Feb 1 2019

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Wilke, A. B. B., Carvajal, A., Medina, J., Anderson, M., Nieves, V. J., Ramirez, M., Vasquez, C., Petrie, W., Cardenas, G., & Beier, J. C. (2019). Assessment of the effectiveness of BG-Sentinel traps baited with CO 2 and BG-Lure for the surveillance of vector mosquitoes in Miami-Dade County, Florida PloS one, 14(2), [e0212688]. https://doi.org/10.1371/journal.pone.0212688