Assessment of perforin expression in peripheral blood lymphocytes in psoriatic patients during exacerbation of disease

L. Prpić, Natasa Strbo, V. Sotošek, F. Gruber, E. R. Podack, D. Rukavina

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13 Scopus citations

Abstract

There are very few data concerning the role played by cell-mediated cytotoxicity, particularly at the molecular level, in the course of psoriasis. Both cytotoxic T lymphocytes (CTL) and natural killer cells contain in their granules the cytolytic protein perforin, a mediator in cell-mediated cytotoxicity reactions. The aim of this study was to analyze perforin expression in various sets and subsets of perforin-positive peripheral blood lymphocytes in 17 patients with chronic psoriasis vulgaris in the exacerbation phase. The results were compared with those of an age- and sex-matched healthy control group (n=21). Perforin (intracellular antigen) and cell surface antigens were detected using the simultaneous double-staining method. We found a significant increase in perforin (P) expression in the patient group for CTL (CD3+P+ cells), which are located mostly in the CD8+ population of T lymphocytes (CDS+P+).

Original languageEnglish
Pages (from-to)14-16
Number of pages3
JournalActa Dermato-Venereologica, Supplement
Issue number211
StatePublished - Dec 1 2000
Externally publishedYes

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Keywords

  • Cytotoxic T lymphocytes
  • NK cells
  • Perforin
  • Psoriasis

ASJC Scopus subject areas

  • Dermatology

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