Assessment of micro-mechanical variations in experimental arteriovenous fistulae using atomic force microscopy

Tyler Laurito, Vivian Sueiras, Natasha Fernandez, Luis A. Escobar, Laisel Martinez, Fotios M Andreopoulos, Loay Salman, Roberto I Vazquez-Padron, Noel Marysa Ziebarth

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: This study presents a method to quantify micro-stiffness variations in experimental arteriovenous fistulae (AVF). Methods: AVF created by anastomosing the superficial epigastric vein to the femoral artery in Sprague-Dawley rats were allowed to remodel for 21 days before being harvested and preserved in culture medium. A custom atomic force microscope was used to measure microvascular stiffness (Young’s modulus) in three areas of the AVF: the inflow artery, the juxta-anastomotic area, and the outflow vein. Morphometric measurements and collagen and elastin contents were also determined. Results: Atomic force microscopy indentation revealed an increased stiffness in the juxta-anastomotic area of the AVF compared to the outflow vein and inflow artery. The juxta-anastomotic area was also significantly stiffer than the contralateral vein. The lack of elasticity (higher Young’s modulus) of the juxta-anastomotic region was associated with a thicker vascular wall that was rich in collagen but poor in elastin. Conclusions: This study demonstrates for the first time the feasibility of using atomic force microscopy to measure local stiffness variations in experimental AVF. This technique could be instrumental in advancing our understanding of how micro-spatial organization of the AVF wall determines the overall biomechanical performance of this type of vascular access.

Original languageEnglish (US)
Pages (from-to)279-283
Number of pages5
JournalJournal of Vascular Access
Volume17
Issue number3
DOIs
StatePublished - May 1 2016

Fingerprint

Atomic Force Microscopy
Arteriovenous Fistula
Veins
Elastin
Elastic Modulus
Blood Vessels
Collagen
Arteries
Elasticity
Femoral Artery
Sprague Dawley Rats
Culture Media

Keywords

  • Arteriovenous fistula
  • Collagen
  • Elastin
  • Vascular access
  • Vascular stiffness

ASJC Scopus subject areas

  • Surgery
  • Nephrology

Cite this

Assessment of micro-mechanical variations in experimental arteriovenous fistulae using atomic force microscopy. / Laurito, Tyler; Sueiras, Vivian; Fernandez, Natasha; Escobar, Luis A.; Martinez, Laisel; Andreopoulos, Fotios M; Salman, Loay; Vazquez-Padron, Roberto I; Ziebarth, Noel Marysa.

In: Journal of Vascular Access, Vol. 17, No. 3, 01.05.2016, p. 279-283.

Research output: Contribution to journalArticle

Laurito, Tyler ; Sueiras, Vivian ; Fernandez, Natasha ; Escobar, Luis A. ; Martinez, Laisel ; Andreopoulos, Fotios M ; Salman, Loay ; Vazquez-Padron, Roberto I ; Ziebarth, Noel Marysa. / Assessment of micro-mechanical variations in experimental arteriovenous fistulae using atomic force microscopy. In: Journal of Vascular Access. 2016 ; Vol. 17, No. 3. pp. 279-283.
@article{29b4d5fa487a416bb407193d9a56be0e,
title = "Assessment of micro-mechanical variations in experimental arteriovenous fistulae using atomic force microscopy",
abstract = "Purpose: This study presents a method to quantify micro-stiffness variations in experimental arteriovenous fistulae (AVF). Methods: AVF created by anastomosing the superficial epigastric vein to the femoral artery in Sprague-Dawley rats were allowed to remodel for 21 days before being harvested and preserved in culture medium. A custom atomic force microscope was used to measure microvascular stiffness (Young’s modulus) in three areas of the AVF: the inflow artery, the juxta-anastomotic area, and the outflow vein. Morphometric measurements and collagen and elastin contents were also determined. Results: Atomic force microscopy indentation revealed an increased stiffness in the juxta-anastomotic area of the AVF compared to the outflow vein and inflow artery. The juxta-anastomotic area was also significantly stiffer than the contralateral vein. The lack of elasticity (higher Young’s modulus) of the juxta-anastomotic region was associated with a thicker vascular wall that was rich in collagen but poor in elastin. Conclusions: This study demonstrates for the first time the feasibility of using atomic force microscopy to measure local stiffness variations in experimental AVF. This technique could be instrumental in advancing our understanding of how micro-spatial organization of the AVF wall determines the overall biomechanical performance of this type of vascular access.",
keywords = "Arteriovenous fistula, Collagen, Elastin, Vascular access, Vascular stiffness",
author = "Tyler Laurito and Vivian Sueiras and Natasha Fernandez and Escobar, {Luis A.} and Laisel Martinez and Andreopoulos, {Fotios M} and Loay Salman and Vazquez-Padron, {Roberto I} and Ziebarth, {Noel Marysa}",
year = "2016",
month = "5",
day = "1",
doi = "10.5301/jva.5000514",
language = "English (US)",
volume = "17",
pages = "279--283",
journal = "Journal of Vascular Access",
issn = "1129-7298",
publisher = "Wichtig Publishing",
number = "3",

}

TY - JOUR

T1 - Assessment of micro-mechanical variations in experimental arteriovenous fistulae using atomic force microscopy

AU - Laurito, Tyler

AU - Sueiras, Vivian

AU - Fernandez, Natasha

AU - Escobar, Luis A.

AU - Martinez, Laisel

AU - Andreopoulos, Fotios M

AU - Salman, Loay

AU - Vazquez-Padron, Roberto I

AU - Ziebarth, Noel Marysa

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Purpose: This study presents a method to quantify micro-stiffness variations in experimental arteriovenous fistulae (AVF). Methods: AVF created by anastomosing the superficial epigastric vein to the femoral artery in Sprague-Dawley rats were allowed to remodel for 21 days before being harvested and preserved in culture medium. A custom atomic force microscope was used to measure microvascular stiffness (Young’s modulus) in three areas of the AVF: the inflow artery, the juxta-anastomotic area, and the outflow vein. Morphometric measurements and collagen and elastin contents were also determined. Results: Atomic force microscopy indentation revealed an increased stiffness in the juxta-anastomotic area of the AVF compared to the outflow vein and inflow artery. The juxta-anastomotic area was also significantly stiffer than the contralateral vein. The lack of elasticity (higher Young’s modulus) of the juxta-anastomotic region was associated with a thicker vascular wall that was rich in collagen but poor in elastin. Conclusions: This study demonstrates for the first time the feasibility of using atomic force microscopy to measure local stiffness variations in experimental AVF. This technique could be instrumental in advancing our understanding of how micro-spatial organization of the AVF wall determines the overall biomechanical performance of this type of vascular access.

AB - Purpose: This study presents a method to quantify micro-stiffness variations in experimental arteriovenous fistulae (AVF). Methods: AVF created by anastomosing the superficial epigastric vein to the femoral artery in Sprague-Dawley rats were allowed to remodel for 21 days before being harvested and preserved in culture medium. A custom atomic force microscope was used to measure microvascular stiffness (Young’s modulus) in three areas of the AVF: the inflow artery, the juxta-anastomotic area, and the outflow vein. Morphometric measurements and collagen and elastin contents were also determined. Results: Atomic force microscopy indentation revealed an increased stiffness in the juxta-anastomotic area of the AVF compared to the outflow vein and inflow artery. The juxta-anastomotic area was also significantly stiffer than the contralateral vein. The lack of elasticity (higher Young’s modulus) of the juxta-anastomotic region was associated with a thicker vascular wall that was rich in collagen but poor in elastin. Conclusions: This study demonstrates for the first time the feasibility of using atomic force microscopy to measure local stiffness variations in experimental AVF. This technique could be instrumental in advancing our understanding of how micro-spatial organization of the AVF wall determines the overall biomechanical performance of this type of vascular access.

KW - Arteriovenous fistula

KW - Collagen

KW - Elastin

KW - Vascular access

KW - Vascular stiffness

UR - http://www.scopus.com/inward/record.url?scp=84966644056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84966644056&partnerID=8YFLogxK

U2 - 10.5301/jva.5000514

DO - 10.5301/jva.5000514

M3 - Article

C2 - 27032456

AN - SCOPUS:84966644056

VL - 17

SP - 279

EP - 283

JO - Journal of Vascular Access

JF - Journal of Vascular Access

SN - 1129-7298

IS - 3

ER -