Assessment of GH releasing hormone activity in Sephadex separated fractions of porcine hypothalamic extracts by hypophyseal portal vessel infusion in the rat

J. Takahara, A. Arimura, Andrew V Schally

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4 Citations (Scopus)

Abstract

Growth hormone releasing hormone (GH RH) activity in Sephadex G 25 fractions of porcine stalk median eminence (SME) extracts was examined in vivo by infusing these samples into a rat hypophyseal portal vessel. The increment of immunoreactive GH levels in the serum was used as the index for GH RH activity. The GH RH activities were found in two different locations: in the early fractions Nos. 3 to 4, and in somewhat retarded fraction No. 7. These GH RH activities were not due to TRH (thyroid releasing hormone), vasopressin, or potassium. The location of luteinizing hormone releasing hormone (LH RH) and prolactin release inhibiting hormone (PR IH) determined in this in vivo system was in agreement with those found in other in vivo and in vitro assay systems for LH RH and PR IH, respectively. These results help validate this assay system.

Original languageEnglish
Pages (from-to)428-434
Number of pages7
JournalActa Endocrinologica
Volume78
Issue number3
StatePublished - Dec 1 1975
Externally publishedYes

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Growth Hormone-Releasing Hormone
Prolactin Release-Inhibiting Factors
Swine
Hormones
Gonadotropin-Releasing Hormone
Median Eminence
Vasopressins
Thyroid Hormones
Potassium
sephadex
Serum

ASJC Scopus subject areas

  • Endocrinology

Cite this

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title = "Assessment of GH releasing hormone activity in Sephadex separated fractions of porcine hypothalamic extracts by hypophyseal portal vessel infusion in the rat",
abstract = "Growth hormone releasing hormone (GH RH) activity in Sephadex G 25 fractions of porcine stalk median eminence (SME) extracts was examined in vivo by infusing these samples into a rat hypophyseal portal vessel. The increment of immunoreactive GH levels in the serum was used as the index for GH RH activity. The GH RH activities were found in two different locations: in the early fractions Nos. 3 to 4, and in somewhat retarded fraction No. 7. These GH RH activities were not due to TRH (thyroid releasing hormone), vasopressin, or potassium. The location of luteinizing hormone releasing hormone (LH RH) and prolactin release inhibiting hormone (PR IH) determined in this in vivo system was in agreement with those found in other in vivo and in vitro assay systems for LH RH and PR IH, respectively. These results help validate this assay system.",
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AB - Growth hormone releasing hormone (GH RH) activity in Sephadex G 25 fractions of porcine stalk median eminence (SME) extracts was examined in vivo by infusing these samples into a rat hypophyseal portal vessel. The increment of immunoreactive GH levels in the serum was used as the index for GH RH activity. The GH RH activities were found in two different locations: in the early fractions Nos. 3 to 4, and in somewhat retarded fraction No. 7. These GH RH activities were not due to TRH (thyroid releasing hormone), vasopressin, or potassium. The location of luteinizing hormone releasing hormone (LH RH) and prolactin release inhibiting hormone (PR IH) determined in this in vivo system was in agreement with those found in other in vivo and in vitro assay systems for LH RH and PR IH, respectively. These results help validate this assay system.

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