Assembling the presynaptic active zone: A characterization of an active zone precursor vesicle

Rong Grace Zhai, Hagit Vardinon-Friedman, Claudia Cases-Langhoff, Birgit Becker, Eckart D. Gundelfinger, Noam E. Ziv, Craig C. Garner

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328 Scopus citations

Abstract

The active zone is a specialized region of the presynaptic plasma membrane where synaptic vesicles dock and fuse. In this study, we have investigated the cellular mechanism underlying the transport and recruitment of the active zone protein Piccolo into nascent synapses. Our results show that Piccolo is transported to nascent synapses on an ∼80 nm dense core granulated vesicle together with other constituents of the active zone, including Bassoon, Syntaxin, SNAP-25, and N-cadherin, as well as chromogranin B. Components of synaptic vesicles, such as VAMP 2/synaptobrevin II, synaptophysin, synaptotagmin, or proteins of the perisynaptic plasma membrane such as GABA transporter 1 (GAT1), were not present. These studies demonstrate that the presynaptic active zone is formed in part by the fusion of an active zone precursor vesicle with the presynaptic plasma membrane.

Original languageEnglish (US)
Pages (from-to)131-143
Number of pages13
JournalNeuron
Volume29
Issue number1
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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    Zhai, R. G., Vardinon-Friedman, H., Cases-Langhoff, C., Becker, B., Gundelfinger, E. D., Ziv, N. E., & Garner, C. C. (2001). Assembling the presynaptic active zone: A characterization of an active zone precursor vesicle. Neuron, 29(1), 131-143. https://doi.org/10.1016/S0896-6273(01)00185-4