Assembling the presynaptic active zone: A characterization of an active zone precursor vesicle

Rong G Zhai, Hagit Vardinon-Friedman, Claudia Cases-Langhoff, Birgit Becker, Eckart D. Gundelfinger, Noam E. Ziv, Craig C. Garner

Research output: Contribution to journalArticle

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Abstract

The active zone is a specialized region of the presynaptic plasma membrane where synaptic vesicles dock and fuse. In this study, we have investigated the cellular mechanism underlying the transport and recruitment of the active zone protein Piccolo into nascent synapses. Our results show that Piccolo is transported to nascent synapses on an ∼80 nm dense core granulated vesicle together with other constituents of the active zone, including Bassoon, Syntaxin, SNAP-25, and N-cadherin, as well as chromogranin B. Components of synaptic vesicles, such as VAMP 2/synaptobrevin II, synaptophysin, synaptotagmin, or proteins of the perisynaptic plasma membrane such as GABA transporter 1 (GAT1), were not present. These studies demonstrate that the presynaptic active zone is formed in part by the fusion of an active zone precursor vesicle with the presynaptic plasma membrane.

Original languageEnglish
Pages (from-to)131-143
Number of pages13
JournalNeuron
Volume29
Issue number1
DOIs
StatePublished - Feb 10 2001
Externally publishedYes

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Synaptic Vesicles
Cell Membrane
Synapses
Synaptotagmin II
Chromogranin B
Vesicle-Associated Membrane Protein 2
R-SNARE Proteins
GABA Plasma Membrane Transport Proteins
Qa-SNARE Proteins
Synaptophysin
Active Biological Transport
Secretory Vesicles
Cadherins
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Zhai, R. G., Vardinon-Friedman, H., Cases-Langhoff, C., Becker, B., Gundelfinger, E. D., Ziv, N. E., & Garner, C. C. (2001). Assembling the presynaptic active zone: A characterization of an active zone precursor vesicle. Neuron, 29(1), 131-143. https://doi.org/10.1016/S0896-6273(01)00185-4

Assembling the presynaptic active zone : A characterization of an active zone precursor vesicle. / Zhai, Rong G; Vardinon-Friedman, Hagit; Cases-Langhoff, Claudia; Becker, Birgit; Gundelfinger, Eckart D.; Ziv, Noam E.; Garner, Craig C.

In: Neuron, Vol. 29, No. 1, 10.02.2001, p. 131-143.

Research output: Contribution to journalArticle

Zhai, RG, Vardinon-Friedman, H, Cases-Langhoff, C, Becker, B, Gundelfinger, ED, Ziv, NE & Garner, CC 2001, 'Assembling the presynaptic active zone: A characterization of an active zone precursor vesicle', Neuron, vol. 29, no. 1, pp. 131-143. https://doi.org/10.1016/S0896-6273(01)00185-4
Zhai RG, Vardinon-Friedman H, Cases-Langhoff C, Becker B, Gundelfinger ED, Ziv NE et al. Assembling the presynaptic active zone: A characterization of an active zone precursor vesicle. Neuron. 2001 Feb 10;29(1):131-143. https://doi.org/10.1016/S0896-6273(01)00185-4
Zhai, Rong G ; Vardinon-Friedman, Hagit ; Cases-Langhoff, Claudia ; Becker, Birgit ; Gundelfinger, Eckart D. ; Ziv, Noam E. ; Garner, Craig C. / Assembling the presynaptic active zone : A characterization of an active zone precursor vesicle. In: Neuron. 2001 ; Vol. 29, No. 1. pp. 131-143.
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