@article{6d5d04ed3d4b41078209741d96c11872,
title = "Assembling OX40 Aptamers on a Molecular Scaffold to Create a Receptor-Activating Aptamer",
abstract = "We show that a molecular scaffold can be utilized to convert a receptor binding aptamer into a receptor agonist. Many receptors (including tumor necrosis receptor family members) are activated when they are multimerized on the cell surface. Molecular scaffolds have been utilized to assemble multiple receptor binding peptide ligands to generate activators of such receptors. We demonstrate that an RNA aptamer that recognizes OX40, a member of the tumor necrosis factor receptor superfamily, can be converted into a receptor-activating aptamer by assembling two copies on an olignucleotide-based scaffold. The OX40 receptor-activating aptamer is able to induce nuclear localization of nuclear factor-κB, cytokine production, and cell proliferation, as well as enhance the potency of dendritic cell-based tumor vaccines when systemically delivered to mice.",
keywords = "CHEMBIO, RNA",
author = "Dollins, {Claudia M.} and Smita Nair and David Boczkowski and Jaewoo Lee and Layzer, {Juliana M.} and Eli Gilboa and Sullenger, {Bruce A.}",
note = "Funding Information: This publication was made possible by grant 1UL1 RR024128-01 from the National Institutes for Research Resources, a component of the National Institutes of Health (NIH), and roadmap for Medical Research to S.N., as well as grant HL065222 from the NIH to B.A.S. We would like to thank D. Snyder, J. Mi, and Y. Liu for their technical assistance, and D.E. Dollins as well as M. Kierlin-Duncan for critical reading of this manuscript. Thanks to B. Ramsay Shaw for critical discussions. B.A.S. and E.G. are scientific founders of Argos Biosciences, a company focused on the clinical development of DC-based vaccines, and B.A.S. is a scientific founder of b3 Bio Inc., a biotechnology company focused upon using aptamers as delivery agents. Neither company provided any support for the studies performed in the manuscript. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",
year = "2008",
month = jul,
day = "21",
doi = "10.1016/j.chembiol.2008.05.016",
language = "English (US)",
volume = "15",
pages = "675--682",
journal = "Cell Chemical Biology",
issn = "2451-9448",
publisher = "Elsevier Inc.",
number = "7",
}