Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae

Ling Zhang; Kristi Jones; Michael J. Smerdon; Feng Gong

doi:10.1016/j.ymeth.2009.03.017

Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae

Ling Zhang, Kristi Jones, Michael J. Smerdon, Feng Gong

Biochemistry & Molecular Biology

Research output: Contribution to journal › Review article › peer-review

5 Scopus citations

Abstract

How DNA repair proteins interact with the dynamic structure of chromatin is an emerging question. Chromatin structure impedes the access of repair proteins to sites of DNA damage. Several recent studies have implicated chromatin remodeling complexes in DNA repair. In this report we summarize the methods we used to investigate chromatin remodeling during nucleotide excision repair (NER) in vivo. We describe a procedure to analyze UV-induced chromatin remodeling at the silent mating-type locus HML using isolated nuclei from UV-treated yeast cells. In addition, a method to capture transient protein-protein associations in chromatin is outlined. We have used the methods described here to demonstrate that the SWI/SNF chromatin remodeling complex is involved in chromatin rearrangement during NER.

Original language	English (US)
Pages (from-to)	19-22
Number of pages	4
Journal	Methods
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/j.ymeth.2009.03.017
State	Published - May 2009

Keywords

Chromatin
Methods
Nucleotide excision repair
SWI/SNF
Yeast

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.ymeth.2009.03.017

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title = "Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae",

abstract = "How DNA repair proteins interact with the dynamic structure of chromatin is an emerging question. Chromatin structure impedes the access of repair proteins to sites of DNA damage. Several recent studies have implicated chromatin remodeling complexes in DNA repair. In this report we summarize the methods we used to investigate chromatin remodeling during nucleotide excision repair (NER) in vivo. We describe a procedure to analyze UV-induced chromatin remodeling at the silent mating-type locus HML using isolated nuclei from UV-treated yeast cells. In addition, a method to capture transient protein-protein associations in chromatin is outlined. We have used the methods described here to demonstrate that the SWI/SNF chromatin remodeling complex is involved in chromatin rearrangement during NER.",

keywords = "Chromatin, Methods, Nucleotide excision repair, SWI/SNF, Yeast",

author = "Ling Zhang and Kristi Jones and Smerdon, {Michael J.} and Feng Gong",

note = "Funding Information: This work was supported, in part, by funds from the CONCERN Foundation, the American Cancer Society and the Sylvester Comprehensive Cancer Center (to F.G.), and NIH Grants ES04106 and ES002614 (to M.J.S.).",

year = "2009",

month = may,

doi = "10.1016/j.ymeth.2009.03.017",

language = "English (US)",

volume = "48",

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journal = "Methods in Enzymology",

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publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae

AU - Zhang, Ling

AU - Jones, Kristi

AU - Smerdon, Michael J.

AU - Gong, Feng

N1 - Funding Information: This work was supported, in part, by funds from the CONCERN Foundation, the American Cancer Society and the Sylvester Comprehensive Cancer Center (to F.G.), and NIH Grants ES04106 and ES002614 (to M.J.S.).

PY - 2009/5

Y1 - 2009/5

N2 - How DNA repair proteins interact with the dynamic structure of chromatin is an emerging question. Chromatin structure impedes the access of repair proteins to sites of DNA damage. Several recent studies have implicated chromatin remodeling complexes in DNA repair. In this report we summarize the methods we used to investigate chromatin remodeling during nucleotide excision repair (NER) in vivo. We describe a procedure to analyze UV-induced chromatin remodeling at the silent mating-type locus HML using isolated nuclei from UV-treated yeast cells. In addition, a method to capture transient protein-protein associations in chromatin is outlined. We have used the methods described here to demonstrate that the SWI/SNF chromatin remodeling complex is involved in chromatin rearrangement during NER.

AB - How DNA repair proteins interact with the dynamic structure of chromatin is an emerging question. Chromatin structure impedes the access of repair proteins to sites of DNA damage. Several recent studies have implicated chromatin remodeling complexes in DNA repair. In this report we summarize the methods we used to investigate chromatin remodeling during nucleotide excision repair (NER) in vivo. We describe a procedure to analyze UV-induced chromatin remodeling at the silent mating-type locus HML using isolated nuclei from UV-treated yeast cells. In addition, a method to capture transient protein-protein associations in chromatin is outlined. We have used the methods described here to demonstrate that the SWI/SNF chromatin remodeling complex is involved in chromatin rearrangement during NER.

KW - Chromatin

KW - Methods

KW - Nucleotide excision repair

KW - SWI/SNF

KW - Yeast

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