Assaying genome-wide recombination and centromere functions with Arabidopsis tetrads

Gregory P. Copenhaver, William Browne, Daphne Preuss

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

During meiosis, crossover events generate new allelic combinations, yet the abundance of these genetic exchanges in individual cells has not been measured previously on a genomic level. To perform a genome-wide analysis of recombination, we monitored the assortment of genetic markers in meiotic tetrads from Arabidopsis. By determining the number and distribution of crossovers in individual meiotic cells, we demonstrated (i) surprisingly precise regulation of crossover number in each meiosis, (ii) considerably reduced recombination along chromosomes carrying ribosomal DNA arrays, and (iii) an inversely proportional relationship between recombination frequencies and chromosome size. This use of tetrad analysis also achieved precise mapping of all five Arabidopsis centromeres, localizing centromere functions in the intact chromosomes of a higher eukaryote.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number1
DOIs
StatePublished - Jan 6 1998
Externally publishedYes

Fingerprint

Centromere
Arabidopsis
Genetic Recombination
Chromosomes
Meiosis
Genome
Ribosomal DNA
Oligonucleotide Array Sequence Analysis
Eukaryota
Genetic Markers

Keywords

  • Crossover interference
  • Meiosis
  • Tetrad analysis

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Assaying genome-wide recombination and centromere functions with Arabidopsis tetrads. / Copenhaver, Gregory P.; Browne, William; Preuss, Daphne.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 1, 06.01.1998, p. 247-252.

Research output: Contribution to journalArticle

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