Ascorbate induces ten-eleven translocation (Tet) methylcytosine dioxygenase-mediated generation of 5-hydroxymethylcytosine

Emily A. Minor, Brenda L. Court, Juan Young, Gaofeng Wang

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Background: Tet methylcytosine dioxygenase converts 5-mC to 5-hmC in DNA. Results: Ascorbate significantly and specifically enhances Tet-mediated generation of 5-hmC. Conclusion: Our findings suggest that ascorbate enhances 5-hmC generation, most likely by acting as a co-factor for Tet methylcytosine dioxygenase to generate 5-hmC. Significance: The availability of ascorbate could have significant consequences for health and diseases by modulating the epigenetic control of genome activity.

Original languageEnglish
Pages (from-to)13669-13674
Number of pages6
JournalJournal of Biological Chemistry
Volume288
Issue number19
DOIs
StatePublished - May 10 2013

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Dioxygenases
Epigenomics
Genes
Health
Availability
Genome
DNA
5-hydroxymethylcytosine

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Ascorbate induces ten-eleven translocation (Tet) methylcytosine dioxygenase-mediated generation of 5-hydroxymethylcytosine. / Minor, Emily A.; Court, Brenda L.; Young, Juan; Wang, Gaofeng.

In: Journal of Biological Chemistry, Vol. 288, No. 19, 10.05.2013, p. 13669-13674.

Research output: Contribution to journalArticle

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AB - Background: Tet methylcytosine dioxygenase converts 5-mC to 5-hmC in DNA. Results: Ascorbate significantly and specifically enhances Tet-mediated generation of 5-hmC. Conclusion: Our findings suggest that ascorbate enhances 5-hmC generation, most likely by acting as a co-factor for Tet methylcytosine dioxygenase to generate 5-hmC. Significance: The availability of ascorbate could have significant consequences for health and diseases by modulating the epigenetic control of genome activity.

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