Artificial nucleases

Chi Hong B. Chen; Lisa Milne; Ralf Landgraf; David M. Perrin; David S. Sigman

doi:10.1002/1439-7633(20011001)2:10<735::aid-cbic735>3.0.co;2-%23

Artificial nucleases

Chi Hong B. Chen, Lisa Milne, Ralf Landgraf, David M. Perrin, David S. Sigman

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(CO) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(CO).

Original language	English (US)
Pages (from-to)	735-740
Number of pages	6
Journal	ChemBioChem
Volume	2
Issue number	10
DOIs	https://doi.org/10.1002/1439-7633(20011001)2:10<735::aid-cbic735>3.0.co;2-%23
State	Published - 2001
Externally published	Yes

Keywords

Copper
DNA cleavage
Dna recognition
Nucleic acids
Oligonucleotides

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Organic Chemistry
Drug Discovery

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10.1002/1439-7633(20011001)2:10<735::aid-cbic735>3.0.co;2-%23

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AU - Milne, Lisa

AU - Landgraf, Ralf

AU - Perrin, David M.

AU - Sigman, David S.

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Y1 - 2001

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AB - The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(CO) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(CO).

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KW - DNA cleavage

KW - Dna recognition

KW - Nucleic acids

KW - Oligonucleotides

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Artificial nucleases

Abstract

Keywords

ASJC Scopus subject areas

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