Abstract
The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(CO) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(CO).
Original language | English (US) |
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Pages (from-to) | 735-740 |
Number of pages | 6 |
Journal | ChemBioChem |
Volume | 2 |
Issue number | 10 |
DOIs | |
State | Published - 2001 |
Externally published | Yes |
Keywords
- Copper
- DNA cleavage
- Dna recognition
- Nucleic acids
- Oligonucleotides
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Organic Chemistry
- Drug Discovery