Artificial nucleases

Chi Hong B Chen, Lisa Milne, Ralf Landgraf, David M. Perrin, David S. Sigman

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(CO) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(CO).

Original languageEnglish
Pages (from-to)735-740
Number of pages6
JournalChemBioChem
Volume2
Issue number10
StatePublished - Dec 1 2001
Externally publishedYes

Fingerprint

Oligonucleotides
DNA
DNA-Binding Proteins
Nucleic Acids
Hydrogen Peroxide
RNA
Oxidation
Proteins
copper-1,10-phenanthroline
1,10-phenanthroline

Keywords

  • Copper
  • DNA cleavage
  • Dna recognition
  • Nucleic acids
  • Oligonucleotides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry
  • Drug Discovery

Cite this

Chen, C. H. B., Milne, L., Landgraf, R., Perrin, D. M., & Sigman, D. S. (2001). Artificial nucleases. ChemBioChem, 2(10), 735-740.

Artificial nucleases. / Chen, Chi Hong B; Milne, Lisa; Landgraf, Ralf; Perrin, David M.; Sigman, David S.

In: ChemBioChem, Vol. 2, No. 10, 01.12.2001, p. 735-740.

Research output: Contribution to journalArticle

Chen, CHB, Milne, L, Landgraf, R, Perrin, DM & Sigman, DS 2001, 'Artificial nucleases', ChemBioChem, vol. 2, no. 10, pp. 735-740.
Chen CHB, Milne L, Landgraf R, Perrin DM, Sigman DS. Artificial nucleases. ChemBioChem. 2001 Dec 1;2(10):735-740.
Chen, Chi Hong B ; Milne, Lisa ; Landgraf, Ralf ; Perrin, David M. ; Sigman, David S. / Artificial nucleases. In: ChemBioChem. 2001 ; Vol. 2, No. 10. pp. 735-740.
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