Abstract
The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(CO) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(CO).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 735-740 |
| Number of pages | 6 |
| Journal | ChemBioChem |
| Volume | 2 |
| Issue number | 10 |
| DOIs | |
| State | Published - Jan 1 2001 |
| Externally published | Yes |
Keywords
- Copper
- DNA cleavage
- Dna recognition
- Nucleic acids
- Oligonucleotides
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Organic Chemistry
- Drug Discovery
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Cite this
Chen, C. H. B., Milne, L., Landgraf, R., Perrin, D. M., & Sigman, D. S. (2001). Artificial nucleases. ChemBioChem, 2(10), 735-740. https://doi.org/10.1002/1439-7633(20011001)2:10<735::aid-cbic735>3.0.co;2-%23