Abstract
The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(CO) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(CO).
| Original language | English |
|---|---|
| Pages (from-to) | 735-740 |
| Number of pages | 6 |
| Journal | ChemBioChem |
| Volume | 2 |
| Issue number | 10 |
| State | Published - Dec 1 2001 |
| Externally published | Yes |
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Keywords
- Copper
- DNA cleavage
- Dna recognition
- Nucleic acids
- Oligonucleotides
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Organic Chemistry
- Drug Discovery
Cite this
Artificial nucleases. / Chen, Chi Hong B; Milne, Lisa; Landgraf, Ralf; Perrin, David M.; Sigman, David S.
In: ChemBioChem, Vol. 2, No. 10, 01.12.2001, p. 735-740.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Artificial nucleases
AU - Chen, Chi Hong B
AU - Milne, Lisa
AU - Landgraf, Ralf
AU - Perrin, David M.
AU - Sigman, David S.
PY - 2001/12/1
Y1 - 2001/12/1
N2 - The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(CO) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(CO).
AB - The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(CO) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(CO).
KW - Copper
KW - DNA cleavage
KW - Dna recognition
KW - Nucleic acids
KW - Oligonucleotides
UR - http://www.scopus.com/inward/record.url?scp=0035495530&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035495530&partnerID=8YFLogxK
M3 - Article
C2 - 11948855
AN - SCOPUS:0035495530
VL - 2
SP - 735
EP - 740
JO - ChemBioChem
JF - ChemBioChem
SN - 1439-4227
IS - 10
ER -