Artificial nucleases

Chi Hong B. Chen, Lisa Milne, Ralf Landgraf, David M. Perrin, David S. Sigman

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(CO) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(CO).

Original languageEnglish (US)
Pages (from-to)735-740
Number of pages6
JournalChemBioChem
Volume2
Issue number10
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Copper
  • DNA cleavage
  • Dna recognition
  • Nucleic acids
  • Oligonucleotides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry
  • Drug Discovery

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