TY - JOUR
T1 - Arsenic toxicity in the human nerve cell line SK-N-SH in the presence of chromium and copper
AU - Hu, Ligang
AU - Greer, Justin B.
AU - Solo-Gabriele, Helena
AU - Fieber, Lynne A.
AU - Cai, Yong
N1 - Funding Information:
This study was partially supported by NIEHS ARCH (S11 ES11181) and NIH-MBRS (3 S06 GM008205-20S1) programs. This is contribution # 598 of Southeast Environmental Research Center at FIU.
PY - 2013/5
Y1 - 2013/5
N2 - As, Cr, and Cu represent one potential combination of multiple metals/metalloids exposures since these three elements are simultaneously leached from chromated copper arsenate (CCA)-treated wood, a common product used for building construction, at levels that can be potentially harmful. This study investigated the neurotoxicity of As associated with CCA-treated wood when accompanied by Cr and Cu. The toxicity was evaluated on the basis of a cytotoxicity model using human neuroblastoma cell line SK-N-SH. The cells were cultured with CCA-treated wood leachates or with solutions containing arsenate [As(V)], divalent copper [Cu(II)], trivalent chromium [Cr(III)] alone or in different combinations of the three elements. The toxicity was evaluated using variations in cell replication compared to controls after 96. h exposure. Among the three elements present in wood leachates, As played the primary role in the observed toxic effects, which were exerted through multiple pathways, including the generation of oxidative stress. DOM affected the absorption of metals/metalloids into the test cells, which however did not obviously appear to impact toxicity. As toxicity was enhanced by Cu(II) and inhibited by Cr(III) at concentrations below U.S. EPA's allowable maximum contaminant levels in drinking waters. Thus assessing As toxicity in real environments is not sufficient if based solely on the result from As.
AB - As, Cr, and Cu represent one potential combination of multiple metals/metalloids exposures since these three elements are simultaneously leached from chromated copper arsenate (CCA)-treated wood, a common product used for building construction, at levels that can be potentially harmful. This study investigated the neurotoxicity of As associated with CCA-treated wood when accompanied by Cr and Cu. The toxicity was evaluated on the basis of a cytotoxicity model using human neuroblastoma cell line SK-N-SH. The cells were cultured with CCA-treated wood leachates or with solutions containing arsenate [As(V)], divalent copper [Cu(II)], trivalent chromium [Cr(III)] alone or in different combinations of the three elements. The toxicity was evaluated using variations in cell replication compared to controls after 96. h exposure. Among the three elements present in wood leachates, As played the primary role in the observed toxic effects, which were exerted through multiple pathways, including the generation of oxidative stress. DOM affected the absorption of metals/metalloids into the test cells, which however did not obviously appear to impact toxicity. As toxicity was enhanced by Cu(II) and inhibited by Cr(III) at concentrations below U.S. EPA's allowable maximum contaminant levels in drinking waters. Thus assessing As toxicity in real environments is not sufficient if based solely on the result from As.
KW - Arsenic
KW - Chromium
KW - Combined toxic effect
KW - Copper
KW - Neurotoxicity
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U2 - 10.1016/j.chemosphere.2013.01.005
DO - 10.1016/j.chemosphere.2013.01.005
M3 - Article
C2 - 23473430
AN - SCOPUS:84876329171
VL - 91
SP - 1082
EP - 1087
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 8
ER -