Arginylation regulates intracellular actin polymer level by modulating actin properties and binding of capping and severing proteins

Sougata Saha, Maureen M. Mundia, Fangliang Zhang, Ryan W. Demers, Farida Korobova, Tatyana Svitkina, Alex A. Perieteanu, John F. Dawson, Anna Kashina

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Actin arginylation regulates lamella formation in motile fibroblasts, but the underlying molecular mechanisms are unknown. To understand how arginylation affects the actin cytoskeleton, we investigated the biochemical properties and the structural organization of actin filaments in wild-type and arginyltransferase (Ate1) knockout cells. We found that Ate1 knockout results in a dramatic reduction of the actin polymer levels in vivo accompanied by a corresponding increase in the monomer level. Purified nonarginylated actin has altered polymerization properties, and actin filaments from Ate1 knockout cells show altered interactions with several associated proteins. Ate1 knockout cells have severe impairment of cytoskeletal organization throughout the cell. Thus, arginylation regulates the ability of actin to form filaments in the whole cell rather than preventing the collapse of preformed actin networks at the cell leading edge as proposed in our previous model. This regulation is achieved through interconnected mechanisms that involve actin polymerization per se and through binding of actin-associated proteins.

Original languageEnglish
Pages (from-to)1350-1361
Number of pages12
JournalMolecular Biology of the Cell
Volume21
Issue number8
DOIs
StatePublished - Apr 15 2010
Externally publishedYes

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Actins
Polymers
Actin Cytoskeleton
Proteins
arginyltransferase
Polymerization
Microfilament Proteins
Fibroblasts

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Arginylation regulates intracellular actin polymer level by modulating actin properties and binding of capping and severing proteins. / Saha, Sougata; Mundia, Maureen M.; Zhang, Fangliang; Demers, Ryan W.; Korobova, Farida; Svitkina, Tatyana; Perieteanu, Alex A.; Dawson, John F.; Kashina, Anna.

In: Molecular Biology of the Cell, Vol. 21, No. 8, 15.04.2010, p. 1350-1361.

Research output: Contribution to journalArticle

Saha, S, Mundia, MM, Zhang, F, Demers, RW, Korobova, F, Svitkina, T, Perieteanu, AA, Dawson, JF & Kashina, A 2010, 'Arginylation regulates intracellular actin polymer level by modulating actin properties and binding of capping and severing proteins', Molecular Biology of the Cell, vol. 21, no. 8, pp. 1350-1361. https://doi.org/10.1091/mbc.E09-09-0829
Saha, Sougata ; Mundia, Maureen M. ; Zhang, Fangliang ; Demers, Ryan W. ; Korobova, Farida ; Svitkina, Tatyana ; Perieteanu, Alex A. ; Dawson, John F. ; Kashina, Anna. / Arginylation regulates intracellular actin polymer level by modulating actin properties and binding of capping and severing proteins. In: Molecular Biology of the Cell. 2010 ; Vol. 21, No. 8. pp. 1350-1361.
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