Abstract
Eel gill lipids were labelled in vivo with (32P) phosphate and (14C) acetate as precursors added to the water in the incubation tank. We compared the transfer of fish from brackish water (BW) to fresh water (FW) and also the transfer from sea water (SW) to FW, with the corresponding transfer from FW to demineralised FW (soft fresh water, SFW). Results show a common (32P) phosphatidylethanolamine (PE) dominated phospholipid incorporation pattern at steady state, whatever environmental salinity the eels are adapted to, be it SW, BW, FW or finally after about a week in SFW. A deviation from any established steady state, by lowering the environmental salinity, leads to a temporary loss of the (32P) PE dominated pattern and this applies equally, whether fish are transferred from a hyper/iso- to a hypo-osmotic medium, or remain in a hypo-osmotic medium. After about 1 week in the transfer media, the original (32P) PE dominated phospholipid pattern is restored. The concomitant incorporation of (14C) acetate into eel gill phospholipids is not affected by the induced environmental changes. It shows a (14C) phosphatidylcholine dominated incorporation pattern throughout.
| Original language | English |
|---|---|
| Pages (from-to) | 57-65 |
| Number of pages | 9 |
| Journal | Environmental Biology of Fishes |
| Volume | 70 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 1 2004 |
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Keywords
- (C) acetate
- (P) phosphate phosphatidylethanolamine
- Freshwater
- Lipid-protein interaction
- Palmitoleic acid
- Phosphatidylcholine
- Soft fresh water
ASJC Scopus subject areas
- Aquatic Science
- Ecology
Cite this
Are membrane lipids involved in osmoregulation? Studies in vivo on the European eel, Anguilla anguilla, after reduced ambient salinity. / Hansen, Heinz J M; Grosell, Martin.
In: Environmental Biology of Fishes, Vol. 70, No. 1, 01.05.2004, p. 57-65.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Are membrane lipids involved in osmoregulation? Studies in vivo on the European eel, Anguilla anguilla, after reduced ambient salinity
AU - Hansen, Heinz J M
AU - Grosell, Martin
PY - 2004/5/1
Y1 - 2004/5/1
N2 - Eel gill lipids were labelled in vivo with (32P) phosphate and (14C) acetate as precursors added to the water in the incubation tank. We compared the transfer of fish from brackish water (BW) to fresh water (FW) and also the transfer from sea water (SW) to FW, with the corresponding transfer from FW to demineralised FW (soft fresh water, SFW). Results show a common (32P) phosphatidylethanolamine (PE) dominated phospholipid incorporation pattern at steady state, whatever environmental salinity the eels are adapted to, be it SW, BW, FW or finally after about a week in SFW. A deviation from any established steady state, by lowering the environmental salinity, leads to a temporary loss of the (32P) PE dominated pattern and this applies equally, whether fish are transferred from a hyper/iso- to a hypo-osmotic medium, or remain in a hypo-osmotic medium. After about 1 week in the transfer media, the original (32P) PE dominated phospholipid pattern is restored. The concomitant incorporation of (14C) acetate into eel gill phospholipids is not affected by the induced environmental changes. It shows a (14C) phosphatidylcholine dominated incorporation pattern throughout.
AB - Eel gill lipids were labelled in vivo with (32P) phosphate and (14C) acetate as precursors added to the water in the incubation tank. We compared the transfer of fish from brackish water (BW) to fresh water (FW) and also the transfer from sea water (SW) to FW, with the corresponding transfer from FW to demineralised FW (soft fresh water, SFW). Results show a common (32P) phosphatidylethanolamine (PE) dominated phospholipid incorporation pattern at steady state, whatever environmental salinity the eels are adapted to, be it SW, BW, FW or finally after about a week in SFW. A deviation from any established steady state, by lowering the environmental salinity, leads to a temporary loss of the (32P) PE dominated pattern and this applies equally, whether fish are transferred from a hyper/iso- to a hypo-osmotic medium, or remain in a hypo-osmotic medium. After about 1 week in the transfer media, the original (32P) PE dominated phospholipid pattern is restored. The concomitant incorporation of (14C) acetate into eel gill phospholipids is not affected by the induced environmental changes. It shows a (14C) phosphatidylcholine dominated incorporation pattern throughout.
KW - (C) acetate
KW - (P) phosphate phosphatidylethanolamine
KW - Freshwater
KW - Lipid-protein interaction
KW - Palmitoleic acid
KW - Phosphatidylcholine
KW - Soft fresh water
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UR - http://www.scopus.com/inward/citedby.url?scp=3543035302&partnerID=8YFLogxK
U2 - 10.1023/B:EBFI.0000022852.25057.62
DO - 10.1023/B:EBFI.0000022852.25057.62
M3 - Article
VL - 70
SP - 57
EP - 65
JO - Environmental Biology of Fishes
JF - Environmental Biology of Fishes
SN - 0378-1909
IS - 1
ER -