Are membrane lipids involved in osmoregulation? Studies in vivo on the European eel, Anguilla anguilla, after reduced ambient salinity

Heinz J M Hansen, Martin Grosell

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Eel gill lipids were labelled in vivo with (32P) phosphate and (14C) acetate as precursors added to the water in the incubation tank. We compared the transfer of fish from brackish water (BW) to fresh water (FW) and also the transfer from sea water (SW) to FW, with the corresponding transfer from FW to demineralised FW (soft fresh water, SFW). Results show a common (32P) phosphatidylethanolamine (PE) dominated phospholipid incorporation pattern at steady state, whatever environmental salinity the eels are adapted to, be it SW, BW, FW or finally after about a week in SFW. A deviation from any established steady state, by lowering the environmental salinity, leads to a temporary loss of the (32P) PE dominated pattern and this applies equally, whether fish are transferred from a hyper/iso- to a hypo-osmotic medium, or remain in a hypo-osmotic medium. After about 1 week in the transfer media, the original (32P) PE dominated phospholipid pattern is restored. The concomitant incorporation of (14C) acetate into eel gill phospholipids is not affected by the induced environmental changes. It shows a (14C) phosphatidylcholine dominated incorporation pattern throughout.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalEnvironmental Biology of Fishes
Volume70
Issue number1
DOIs
StatePublished - May 1 2004

Fingerprint

osmoregulation
Anguilla anguilla
eel
in vivo studies
lipid
salinity
membrane
lipids
phosphatidylethanolamines
phospholipid
phospholipids
water
brackish water
gills
seawater
acetates
acetate
fish
phosphatidylcholines
environmental change

Keywords

  • (C) acetate
  • (P) phosphate phosphatidylethanolamine
  • Freshwater
  • Lipid-protein interaction
  • Palmitoleic acid
  • Phosphatidylcholine
  • Soft fresh water

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

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title = "Are membrane lipids involved in osmoregulation? Studies in vivo on the European eel, Anguilla anguilla, after reduced ambient salinity",
abstract = "Eel gill lipids were labelled in vivo with (32P) phosphate and (14C) acetate as precursors added to the water in the incubation tank. We compared the transfer of fish from brackish water (BW) to fresh water (FW) and also the transfer from sea water (SW) to FW, with the corresponding transfer from FW to demineralised FW (soft fresh water, SFW). Results show a common (32P) phosphatidylethanolamine (PE) dominated phospholipid incorporation pattern at steady state, whatever environmental salinity the eels are adapted to, be it SW, BW, FW or finally after about a week in SFW. A deviation from any established steady state, by lowering the environmental salinity, leads to a temporary loss of the (32P) PE dominated pattern and this applies equally, whether fish are transferred from a hyper/iso- to a hypo-osmotic medium, or remain in a hypo-osmotic medium. After about 1 week in the transfer media, the original (32P) PE dominated phospholipid pattern is restored. The concomitant incorporation of (14C) acetate into eel gill phospholipids is not affected by the induced environmental changes. It shows a (14C) phosphatidylcholine dominated incorporation pattern throughout.",
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author = "Hansen, {Heinz J M} and Martin Grosell",
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AU - Grosell, Martin

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