Rat chloroleukemic cells as well as turpentine-induced rat hyperplastic bone marrow cells in culture medium incorporated approximately 63% of [1- 14C]arachidonic acid added to the medium into their cellular membrane phospholipids. Separation of the cellular phospholipid fraction by thin-layer chromatography revealed that most of the [ 14C]arachidonic acid was incorporated into phosphatidylcholine (48%) and phosphatidylethanolamine (12%). The rest of the 3% radioactivity was distributed in sphingomyelin, phosphatidylserine, phosphoinositides, plasmalogen, and phosphatidic acid. Metabolism of [ 14C]arachidonic acid by the chloroleukemic cells and hyperplastic marrow cells during a six-hr quiescent period in culture resulted in a marked transformation of the labeled fatty acid into prostaglandin D 2. Biosynthesis of prostaglandin D 2 statistically (p<0.005) greater in the chloroleukemic cells than in the hyperplastic marrow cells during this nonproliferating phase of both cell types. Biosynthesis of prostaglandin F 2(α) (PGF 2(}) by both cells was minimal during this period. However, 24 hr after both cells had been incubated in culture, the chloroleukemic cells exhibited an almost linear increase in growth, whereas the hyperplastic marrow cells remained quiescent. Biosynthesis of [ 14C]prostaglandin D 2 by both cell types decreased markedly when compared to the six hr period, whereas the biosynthesis of [ 14C]PGF 2(α) by the rapidly proliferating chloroleukemic cells increased markedly. No significant alteration in PGF 2(α) biosynthesis occurred in the quiescent hyperplastic marrow cells. For instance, a statistically significant (p<0.001) amount of extracted 14C in the prostaglandin fraction was associated with PGF 2(α) formed by the chloroleukemic cells when compared to the hyperplastic marrow cells. These results suggest that the increased formation of PGF 2(α) associated with the rapidly proliferating chloroleukemic cells in culture may reflect, at least in part, a property of the rapidly growing cells. Whether these results relate to the abnormal hyperproliferative nature of these cells remains to be determined.
|Original language||English (US)|
|Number of pages||6|
|State||Published - Jan 1 1981|
ASJC Scopus subject areas
- Cancer Research