Abstract
A general strategy was developed to transform aptamers into highly sensitive and selective optical sensors. This was accomplished with an unmodified aptamer as the molecular recognition element and a competitor oligo, which was completely or partially complementary to the aptamer, as the signal transduction element. Displacement of competitor oligos in the presence of targets resulted in a detectable optical readout. Successful application of this strategy has been demonstrated not only with different types of targets (small molecules and proteins), but also with different types of aptamers (DNA and RNA). This strategy has high potential for various applications in high-throughput drug screening and intracellular studies.
| Original language | English |
|---|---|
| Pages (from-to) | 2380-2381 |
| Number of pages | 2 |
| Journal | Journal of the American Chemical Society |
| Volume | 130 |
| Issue number | 8 |
| DOIs | |
| State | Published - Feb 27 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Chemistry(all)
Cite this
Aptamer-based optical probes with separated molecular recognition and signal transduction modules. / Li, Na; Ho, Chih Ming.
In: Journal of the American Chemical Society, Vol. 130, No. 8, 27.02.2008, p. 2380-2381.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aptamer-based optical probes with separated molecular recognition and signal transduction modules
AU - Li, Na
AU - Ho, Chih Ming
PY - 2008/2/27
Y1 - 2008/2/27
N2 - A general strategy was developed to transform aptamers into highly sensitive and selective optical sensors. This was accomplished with an unmodified aptamer as the molecular recognition element and a competitor oligo, which was completely or partially complementary to the aptamer, as the signal transduction element. Displacement of competitor oligos in the presence of targets resulted in a detectable optical readout. Successful application of this strategy has been demonstrated not only with different types of targets (small molecules and proteins), but also with different types of aptamers (DNA and RNA). This strategy has high potential for various applications in high-throughput drug screening and intracellular studies.
AB - A general strategy was developed to transform aptamers into highly sensitive and selective optical sensors. This was accomplished with an unmodified aptamer as the molecular recognition element and a competitor oligo, which was completely or partially complementary to the aptamer, as the signal transduction element. Displacement of competitor oligos in the presence of targets resulted in a detectable optical readout. Successful application of this strategy has been demonstrated not only with different types of targets (small molecules and proteins), but also with different types of aptamers (DNA and RNA). This strategy has high potential for various applications in high-throughput drug screening and intracellular studies.
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UR - http://www.scopus.com/inward/citedby.url?scp=39749179700&partnerID=8YFLogxK
U2 - 10.1021/ja076787b
DO - 10.1021/ja076787b
M3 - Article
C2 - 18247609
AN - SCOPUS:39749179700
VL - 130
SP - 2380
EP - 2381
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 8
ER -