Aptamer-based optical probes with separated molecular recognition and signal transduction modules

Na Li, Chih Ming Ho

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

A general strategy was developed to transform aptamers into highly sensitive and selective optical sensors. This was accomplished with an unmodified aptamer as the molecular recognition element and a competitor oligo, which was completely or partially complementary to the aptamer, as the signal transduction element. Displacement of competitor oligos in the presence of targets resulted in a detectable optical readout. Successful application of this strategy has been demonstrated not only with different types of targets (small molecules and proteins), but also with different types of aptamers (DNA and RNA). This strategy has high potential for various applications in high-throughput drug screening and intracellular studies.

Original languageEnglish
Pages (from-to)2380-2381
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number8
DOIs
StatePublished - Feb 27 2008
Externally publishedYes

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Nucleotide Aptamers
Molecular recognition
Signal transduction
Signal Transduction
Preclinical Drug Evaluations
Optical sensors
RNA
Screening
DNA
Throughput
Proteins
Molecules
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Aptamer-based optical probes with separated molecular recognition and signal transduction modules. / Li, Na; Ho, Chih Ming.

In: Journal of the American Chemical Society, Vol. 130, No. 8, 27.02.2008, p. 2380-2381.

Research output: Contribution to journalArticle

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