Application of tissue clearing and light sheet fluorescence microscopy to assess optic nerve regeneration in unsectioned tissues

Xueting Luo, Benjamin Yungher, Kevin K. Park

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Optic nerve crush injury, as a model to study central nervous system (CNS) injury, is widely used to assess potential therapeutic strategies, aimed at promoting axon regeneration and neuronal survival. Traditional methods to evaluate optic nerve regeneration rely on histological sectioning. However, tissue sectioning results in inevitable loss of three-dimensional (3D) information, such as axonal trajectories and terminations. Here we describe a protocol for whole-tissue assessment of optic nerve regeneration in adult mice without the need for histological sectioning.

Original languageEnglish (US)
Pages (from-to)209-217
Number of pages9
JournalMethods in Molecular Biology
Volume1162
DOIs
StatePublished - 2014
Externally publishedYes

Keywords

  • Axon regeneration
  • Light sheet fluorescence microscopy
  • Optical clearing
  • PTEN
  • Retinal ganglion cells
  • Tissue clearing
  • Ultramicroscope

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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