Abstract
New alternative laboratory means are needed to improve the options for antemortem diagnosis of avian aspergillosis. In this study, 3-hydroxybutyrate was measured in plasma samples collected from a cohort of African penguins (Spheniscus demersus) maintained under human care. Results were interpreted in combination with those of protein electrophoresis and compared with anti-Aspergillus antibody and galactomannan antigen detection. Overall, 3-hydroxybutyrate levels were found significantly increased in Aspergillus-diseased cases versus the control penguin group (P = 0.002). Mean absolute concentration of β-globulins was increased >20% in samples from infected birds, and α2-globublins were also found to be significantly increased versus clinically normal controls (P < 0.001 and P = 0.001 respectively). Of note, the α2-globulins were also significantly increased versus penguins with inflammatory (non-aspergillosis) diseases (P = 0.001). The specificity of 3-hydroxybutyrate, β-globulins, and α2-globulins for aspergillosis was 78.6%, 79.6%, and 92.2%, respectively. Using these measures in tandem resulted in high specificity (>90%) and negative predictive value (≥80%). In contrast, anti-Aspergillus antibody and galactomannan antigen did not distinguish between infected cases and controls (P > 0.05). This study demonstrates that basic testing in tandem with the new biomarker 3-hydroxybutyrate may provide reliable evidence for the diagnosis of aspergillosis in penguins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 696-703 |
| Number of pages | 8 |
| Journal | Journal of Zoo and Wildlife Medicine |
| Volume | 49 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 2018 |
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Keywords
- Aspergillosis
- Aspergillus
- Diagnostic assay
- Electrophoresis
- Hydroxybutyrate
- Penguin
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
Cite this
Application of 3-hydroxybutyrate measurement and plasma protein electrophoresis in the diagnosis of aspergillosis in African penguins (spheniscus demersus). / Desoubeaux, Guillaume; Rodriguez, Marilyn; Bronson, Ellen; Sirpenski, Gayle; Cray, Carolyn.
In: Journal of Zoo and Wildlife Medicine, Vol. 49, No. 3, 01.09.2018, p. 696-703.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Application of 3-hydroxybutyrate measurement and plasma protein electrophoresis in the diagnosis of aspergillosis in African penguins (spheniscus demersus)
AU - Desoubeaux, Guillaume
AU - Rodriguez, Marilyn
AU - Bronson, Ellen
AU - Sirpenski, Gayle
AU - Cray, Carolyn
PY - 2018/9/1
Y1 - 2018/9/1
N2 - New alternative laboratory means are needed to improve the options for antemortem diagnosis of avian aspergillosis. In this study, 3-hydroxybutyrate was measured in plasma samples collected from a cohort of African penguins (Spheniscus demersus) maintained under human care. Results were interpreted in combination with those of protein electrophoresis and compared with anti-Aspergillus antibody and galactomannan antigen detection. Overall, 3-hydroxybutyrate levels were found significantly increased in Aspergillus-diseased cases versus the control penguin group (P = 0.002). Mean absolute concentration of β-globulins was increased >20% in samples from infected birds, and α2-globublins were also found to be significantly increased versus clinically normal controls (P < 0.001 and P = 0.001 respectively). Of note, the α2-globulins were also significantly increased versus penguins with inflammatory (non-aspergillosis) diseases (P = 0.001). The specificity of 3-hydroxybutyrate, β-globulins, and α2-globulins for aspergillosis was 78.6%, 79.6%, and 92.2%, respectively. Using these measures in tandem resulted in high specificity (>90%) and negative predictive value (≥80%). In contrast, anti-Aspergillus antibody and galactomannan antigen did not distinguish between infected cases and controls (P > 0.05). This study demonstrates that basic testing in tandem with the new biomarker 3-hydroxybutyrate may provide reliable evidence for the diagnosis of aspergillosis in penguins.
AB - New alternative laboratory means are needed to improve the options for antemortem diagnosis of avian aspergillosis. In this study, 3-hydroxybutyrate was measured in plasma samples collected from a cohort of African penguins (Spheniscus demersus) maintained under human care. Results were interpreted in combination with those of protein electrophoresis and compared with anti-Aspergillus antibody and galactomannan antigen detection. Overall, 3-hydroxybutyrate levels were found significantly increased in Aspergillus-diseased cases versus the control penguin group (P = 0.002). Mean absolute concentration of β-globulins was increased >20% in samples from infected birds, and α2-globublins were also found to be significantly increased versus clinically normal controls (P < 0.001 and P = 0.001 respectively). Of note, the α2-globulins were also significantly increased versus penguins with inflammatory (non-aspergillosis) diseases (P = 0.001). The specificity of 3-hydroxybutyrate, β-globulins, and α2-globulins for aspergillosis was 78.6%, 79.6%, and 92.2%, respectively. Using these measures in tandem resulted in high specificity (>90%) and negative predictive value (≥80%). In contrast, anti-Aspergillus antibody and galactomannan antigen did not distinguish between infected cases and controls (P > 0.05). This study demonstrates that basic testing in tandem with the new biomarker 3-hydroxybutyrate may provide reliable evidence for the diagnosis of aspergillosis in penguins.
KW - Aspergillosis
KW - Aspergillus
KW - Diagnostic assay
KW - Electrophoresis
KW - Hydroxybutyrate
KW - Penguin
UR - http://www.scopus.com/inward/record.url?scp=85053468203&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053468203&partnerID=8YFLogxK
U2 - 10.1638/2017-0172.1
DO - 10.1638/2017-0172.1
M3 - Article
C2 - 30212328
AN - SCOPUS:85053468203
VL - 49
SP - 696
EP - 703
JO - Journal of Zoo and Wildlife Medicine
JF - Journal of Zoo and Wildlife Medicine
SN - 1042-7260
IS - 3
ER -