Application of 3-hydroxybutyrate measurement and plasma protein electrophoresis in the diagnosis of aspergillosis in African penguins (spheniscus demersus)

Guillaume Desoubeaux, Marilyn Rodriguez, Ellen Bronson, Gayle Sirpenski, Carolyn Cray

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

New alternative laboratory means are needed to improve the options for antemortem diagnosis of avian aspergillosis. In this study, 3-hydroxybutyrate was measured in plasma samples collected from a cohort of African penguins (Spheniscus demersus) maintained under human care. Results were interpreted in combination with those of protein electrophoresis and compared with anti-Aspergillus antibody and galactomannan antigen detection. Overall, 3-hydroxybutyrate levels were found significantly increased in Aspergillus-diseased cases versus the control penguin group (P = 0.002). Mean absolute concentration of β-globulins was increased >20% in samples from infected birds, and α2-globublins were also found to be significantly increased versus clinically normal controls (P < 0.001 and P = 0.001 respectively). Of note, the α2-globulins were also significantly increased versus penguins with inflammatory (non-aspergillosis) diseases (P = 0.001). The specificity of 3-hydroxybutyrate, β-globulins, and α2-globulins for aspergillosis was 78.6%, 79.6%, and 92.2%, respectively. Using these measures in tandem resulted in high specificity (>90%) and negative predictive value (≥80%). In contrast, anti-Aspergillus antibody and galactomannan antigen did not distinguish between infected cases and controls (P > 0.05). This study demonstrates that basic testing in tandem with the new biomarker 3-hydroxybutyrate may provide reliable evidence for the diagnosis of aspergillosis in penguins.

Original languageEnglish (US)
Pages (from-to)696-703
Number of pages8
JournalJournal of Zoo and Wildlife Medicine
Volume49
Issue number3
DOIs
StatePublished - Sep 1 2018

Fingerprint

Spheniscidae
3-hydroxybutyric acid
Aspergillosis
3-Hydroxybutyric Acid
aspergillosis
Aspergillus
blood proteins
electrophoresis
Electrophoresis
Blood Proteins
penguins
Anti-Idiotypic Antibodies
Antigens
antigen detection
antibodies
Globulins
globulins
Birds
biomarkers
Biomarkers

Keywords

  • Aspergillosis
  • Aspergillus
  • Diagnostic assay
  • Electrophoresis
  • Hydroxybutyrate
  • Penguin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Application of 3-hydroxybutyrate measurement and plasma protein electrophoresis in the diagnosis of aspergillosis in African penguins (spheniscus demersus). / Desoubeaux, Guillaume; Rodriguez, Marilyn; Bronson, Ellen; Sirpenski, Gayle; Cray, Carolyn.

In: Journal of Zoo and Wildlife Medicine, Vol. 49, No. 3, 01.09.2018, p. 696-703.

Research output: Contribution to journalArticle

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