Apparent non-involvement of transfer RNA nucleotidyltransferase in the biosynthesis of Escherichia coli suppressor transfer RNAs

Jeffrey W. Morse, Murray P. Deutscher

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Transfer RNA nucleotidyltransferase has previously been shown to be required for the normal growth of Escherichia coli and for the biosynthesis of some bacteriophage T4 tRNAs. In order to obtain information about the involvement of this enzyme in E. coli tRNA biosynthesis we have measured the level of activity of suppressors 1 to 6 in strains carrying either a cca+ or cca allele. We found that cca strains, deficient in tRNA nucleotidyltransferase, contained the same amount of suppressor activities as the wild-type cca+ strains as determined by suppression of nonsense mutations in both E. coli alkaline phosphatase and in genes of bacteriophage T4. The results suggest that tRNA nucleotidyltransferase is not required for the biosynthesis of tRNAs specified by suppressors 1 to 6.

Original languageEnglish (US)
Pages (from-to)141-144
Number of pages4
JournalJournal of molecular biology
Volume95
Issue number1
DOIs
StatePublished - Jun 15 1975

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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