Apoptotic and antiapoptotic mechanisms after traumatic brain injury

Robert Keane, Susan Kraydieh, George Lotocki, Ofelia F. Alonso, Phillip Aldana, W. Dalton Dietrich

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Caspase and inhibitor of apoptosis (IAP) expression was examined in rats subjected to moderate traumatic brain injury (TBI) using a parasagittal fluid-percussion brain insult (1.7 to 2.2 atm). Within 1 hour after injury, caspase-8 and -9, two initiators of apoptosis, were predominantly expressed in superficial cortical areas adjacent to the impact site and in the thalamus. Caspase-3, an effector caspase, was evident at 6 hours throughout the traumatized cerebral cortex and hippocampus. Moreover, the authors observed that XIAP, cIAP-1, and cIAP-2, members of the IAP family, were constitutively expressed in the brain. Colocalization of XIAP-immunolabled cells with cell-specific markers indicated that XIAP is expressed within neurons and a subpopulation of oligodendrocytes, Immunoblots of brain extracts revealed that the processed forms of caspase-8, -9, and -3 are present as early as 1 hour after trauma. The appearance of activated caspases corresponded with the detection of cleavage of XIAP into fragments after injury and a concomitant increase in the levels of cIAP-1 and cIAP-2 in the traumatized hemispheres. The current data are consistent with the hypotheses that caspases in both the extrinsic and intrinsic apoptotic pathways are activated after moderate TBI and that IAPs may have a protective role within the brain with alterations in levels and cleavage of IAPs that contribute to cell death in this setting.

Original languageEnglish
Pages (from-to)1189-1198
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume21
Issue number10
StatePublished - Oct 20 2001

Fingerprint

Caspase 9
Caspase 8
Brain
Apoptosis
Caspases
Wounds and Injuries
Effector Caspases
Percussion
Caspase Inhibitors
Oligodendroglia
Thalamus
Caspase 3
Cerebral Cortex
Hippocampus
Cell Death
Neurons
Traumatic Brain Injury

Keywords

  • Apoptosis
  • Caspases
  • IAPs
  • Traumatic brain injury.

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Apoptotic and antiapoptotic mechanisms after traumatic brain injury. / Keane, Robert; Kraydieh, Susan; Lotocki, George; Alonso, Ofelia F.; Aldana, Phillip; Dalton Dietrich, W.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 21, No. 10, 20.10.2001, p. 1189-1198.

Research output: Contribution to journalArticle

Keane, Robert ; Kraydieh, Susan ; Lotocki, George ; Alonso, Ofelia F. ; Aldana, Phillip ; Dalton Dietrich, W. / Apoptotic and antiapoptotic mechanisms after traumatic brain injury. In: Journal of Cerebral Blood Flow and Metabolism. 2001 ; Vol. 21, No. 10. pp. 1189-1198.
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