Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2

T. Ohmori, E. R. Podack, K. Nishio, M. Takahashi, Y. Miyahara, Y. Takeda, N. Kubota, Y. Funayama, H. Ogasawara, T. Ohira, S. Ohta, N. Saijo

Research output: Contribution to journalArticle

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Abstract

To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.

Original languageEnglish
Pages (from-to)30-36
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume192
Issue number1
DOIs
StatePublished - Jul 15 1993
Externally publishedYes

Fingerprint

irinotecan
Delta modulation
DNA Fragmentation
Lung Neoplasms
Cells
Apoptosis
DNA
Nimustine
Neoplasms
Agar Gel Electrophoresis
Small Cell Lung Carcinoma
Etoposide
Cell death
Cytotoxicity
Paclitaxel
Electrophoresis
Sepharose
Cell Death
Gels
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2. / Ohmori, T.; Podack, E. R.; Nishio, K.; Takahashi, M.; Miyahara, Y.; Takeda, Y.; Kubota, N.; Funayama, Y.; Ogasawara, H.; Ohira, T.; Ohta, S.; Saijo, N.

In: Biochemical and Biophysical Research Communications, Vol. 192, No. 1, 15.07.1993, p. 30-36.

Research output: Contribution to journalArticle

Ohmori, T, Podack, ER, Nishio, K, Takahashi, M, Miyahara, Y, Takeda, Y, Kubota, N, Funayama, Y, Ogasawara, H, Ohira, T, Ohta, S & Saijo, N 1993, 'Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2', Biochemical and Biophysical Research Communications, vol. 192, no. 1, pp. 30-36. https://doi.org/10.1006/bbrc.1993.1377
Ohmori, T. ; Podack, E. R. ; Nishio, K. ; Takahashi, M. ; Miyahara, Y. ; Takeda, Y. ; Kubota, N. ; Funayama, Y. ; Ogasawara, H. ; Ohira, T. ; Ohta, S. ; Saijo, N. / Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 192, No. 1. pp. 30-36.
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