Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2

T. Ohmori; E. R. Podack; K. Nishio; M. Takahashi; Y. Miyahara; Y. Takeda; N. Kubota; Y. Funayama; H. Ogasawara; T. Ohira; S. Ohta; N. Saijo

doi:10.1006/bbrc.1993.1377

Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2

T. Ohmori, E. R. Podack, K. Nishio, M. Takahashi, Y. Miyahara, Y. Takeda, N. Kubota, Y. Funayama, H. Ogasawara, T. Ohira, S. Ohta, N. Saijo

Research output: Contribution to journal › Article

165 Scopus citations

Abstract

To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.

Original language	English
Pages (from-to)	30-36
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	192
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1993.1377
State	Published - Jul 15 1993
Externally published	Yes

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ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Ohmori, T., Podack, E. R., Nishio, K., Takahashi, M., Miyahara, Y., Takeda, Y., Kubota, N., Funayama, Y., Ogasawara, H., Ohira, T., Ohta, S., & Saijo, N. (1993). Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2. Biochemical and Biophysical Research Communications, 192(1), 30-36. https://doi.org/10.1006/bbrc.1993.1377

Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2. / Ohmori, T.; Podack, E. R.; Nishio, K.; Takahashi, M.; Miyahara, Y.; Takeda, Y.; Kubota, N.; Funayama, Y.; Ogasawara, H.; Ohira, T.; Ohta, S.; Saijo, N.

In: Biochemical and Biophysical Research Communications, Vol. 192, No. 1, 15.07.1993, p. 30-36.

Research output: Contribution to journal › Article

Ohmori, T, Podack, ER, Nishio, K, Takahashi, M, Miyahara, Y, Takeda, Y, Kubota, N, Funayama, Y, Ogasawara, H, Ohira, T, Ohta, S & Saijo, N 1993, 'Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2', Biochemical and Biophysical Research Communications, vol. 192, no. 1, pp. 30-36. https://doi.org/10.1006/bbrc.1993.1377

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10.1006/bbrc.1993.1377