Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2

Tohru Ohmori; Eckhard R. Podack; Kazuto Nishio; Minako Takahashi; Yuki Miyahara; Yuichiro Takeda; Naohiro Kubota; Yasunori Funayama; Hayato Ogasawara; Tatsuo Ohira; Sei Ohta; Nagahiro Saijo

doi:10.1006/bbrc.1993.1377

Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2

Tohru Ohmori, Eckhard R. Podack, Kazuto Nishio, Minako Takahashi, Yuki Miyahara, Yuichiro Takeda, Naohiro Kubota, Yasunori Funayama, Hayato Ogasawara, Tatsuo Ohira, Sei Ohta, Nagahiro Saijo

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

166 Scopus citations

Abstract

To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.

Original language	English (US)
Pages (from-to)	30-36
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	192
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1993.1377
State	Published - 1993

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1993.1377

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Ohmori, T., Podack, E. R., Nishio, K., Takahashi, M., Miyahara, Y., Takeda, Y., Kubota, N., Funayama, Y., Ogasawara, H., Ohira, T., Ohta, S., & Saijo, N. (1993). Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2. Biochemical and biophysical research communications, 192(1), 30-36. https://doi.org/10.1006/bbrc.1993.1377

Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2. / Ohmori, Tohru; Podack, Eckhard R.; Nishio, Kazuto; Takahashi, Minako; Miyahara, Yuki; Takeda, Yuichiro; Kubota, Naohiro; Funayama, Yasunori; Ogasawara, Hayato; Ohira, Tatsuo; Ohta, Sei; Saijo, Nagahiro.

In: Biochemical and biophysical research communications, Vol. 192, No. 1, 1993, p. 30-36.

Research output: Contribution to journal › Article › peer-review

Ohmori, T, Podack, ER, Nishio, K, Takahashi, M, Miyahara, Y, Takeda, Y, Kubota, N, Funayama, Y, Ogasawara, H, Ohira, T, Ohta, S & Saijo, N 1993, 'Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2', Biochemical and biophysical research communications, vol. 192, no. 1, pp. 30-36. https://doi.org/10.1006/bbrc.1993.1377

Ohmori, Tohru ; Podack, Eckhard R. ; Nishio, Kazuto ; Takahashi, Minako ; Miyahara, Yuki ; Takeda, Yuichiro ; Kubota, Naohiro ; Funayama, Yasunori ; Ogasawara, Hayato ; Ohira, Tatsuo ; Ohta, Sei ; Saijo, Nagahiro. / Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2. In: Biochemical and biophysical research communications. 1993 ; Vol. 192, No. 1. pp. 30-36.

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abstract = "To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.",

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AU - Nishio, Kazuto

AU - Takahashi, Minako

AU - Miyahara, Yuki

AU - Takeda, Yuichiro

AU - Kubota, Naohiro

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AU - Ohira, Tatsuo

AU - Ohta, Sei

AU - Saijo, Nagahiro

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N2 - To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.

AB - To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.

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Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2

Abstract

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