Abstract
To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.
| Original language | English |
|---|---|
| Pages (from-to) | 30-36 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 192 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 15 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2. / Ohmori, T.; Podack, E. R.; Nishio, K.; Takahashi, M.; Miyahara, Y.; Takeda, Y.; Kubota, N.; Funayama, Y.; Ogasawara, H.; Ohira, T.; Ohta, S.; Saijo, N.
In: Biochemical and Biophysical Research Communications, Vol. 192, No. 1, 15.07.1993, p. 30-36.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by BCL-2
AU - Ohmori, T.
AU - Podack, E. R.
AU - Nishio, K.
AU - Takahashi, M.
AU - Miyahara, Y.
AU - Takeda, Y.
AU - Kubota, N.
AU - Funayama, Y.
AU - Ogasawara, H.
AU - Ohira, T.
AU - Ohta, S.
AU - Saijo, N.
PY - 1993/7/15
Y1 - 1993/7/15
N2 - To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.
AB - To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.
UR - http://www.scopus.com/inward/record.url?scp=0027162207&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027162207&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.1377
DO - 10.1006/bbrc.1993.1377
M3 - Article
C2 - 8476431
AN - SCOPUS:0027162207
VL - 192
SP - 30
EP - 36
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -