Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2

Tohru Ohmori; Eckhard R. Podack; Kazuto Nishio; Minako Takahashi; Yuki Miyahara; Yuichiro Takeda; Naohiro Kubota; Yasunori Funayama; Hayato Ogasawara; Tatsuo Ohira; Sei Ohta; Nagahiro Saijo

doi:10.1006/bbrc.1993.1377

Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2

Tohru Ohmori, Eckhard R. Podack, Kazuto Nishio, Minako Takahashi, Yuki Miyahara, Yuichiro Takeda, Naohiro Kubota, Yasunori Funayama, Hayato Ogasawara, Tatsuo Ohira, Sei Ohta, Nagahiro Saijo

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

166 Scopus citations

Abstract

To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.

Original language	English (US)
Pages (from-to)	30-36
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	192
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1993.1377
State	Published - 1993

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.1993.1377

Fingerprint Dive into the research topics of 'Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2'. Together they form a unique fingerprint.

Cite this

Ohmori, T., Podack, E. R., Nishio, K., Takahashi, M., Miyahara, Y., Takeda, Y., Kubota, N., Funayama, Y., Ogasawara, H., Ohira, T., Ohta, S., & Saijo, N. (1993). Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2. Biochemical and biophysical research communications, 192(1), 30-36. https://doi.org/10.1006/bbrc.1993.1377

Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2. / Ohmori, Tohru; Podack, Eckhard R.; Nishio, Kazuto; Takahashi, Minako; Miyahara, Yuki; Takeda, Yuichiro; Kubota, Naohiro; Funayama, Yasunori; Ogasawara, Hayato; Ohira, Tatsuo; Ohta, Sei; Saijo, Nagahiro.

In: Biochemical and biophysical research communications, Vol. 192, No. 1, 1993, p. 30-36.

Research output: Contribution to journal › Article › peer-review

Ohmori, T, Podack, ER, Nishio, K, Takahashi, M, Miyahara, Y, Takeda, Y, Kubota, N, Funayama, Y, Ogasawara, H, Ohira, T, Ohta, S & Saijo, N 1993, 'Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2', Biochemical and biophysical research communications, vol. 192, no. 1, pp. 30-36. https://doi.org/10.1006/bbrc.1993.1377

Ohmori, Tohru ; Podack, Eckhard R. ; Nishio, Kazuto ; Takahashi, Minako ; Miyahara, Yuki ; Takeda, Yuichiro ; Kubota, Naohiro ; Funayama, Yasunori ; Ogasawara, Hayato ; Ohira, Tatsuo ; Ohta, Sei ; Saijo, Nagahiro. / Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2. In: Biochemical and biophysical research communications. 1993 ; Vol. 192, No. 1. pp. 30-36.

@article{ee8019bd409449908630abef15922311,

title = "Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2",

abstract = "To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.",

author = "Tohru Ohmori and Podack, {Eckhard R.} and Kazuto Nishio and Minako Takahashi and Yuki Miyahara and Yuichiro Takeda and Naohiro Kubota and Yasunori Funayama and Hayato Ogasawara and Tatsuo Ohira and Sei Ohta and Nagahiro Saijo",

year = "1993",

doi = "10.1006/bbrc.1993.1377",

language = "English (US)",

volume = "192",

pages = "30--36",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Apoptosis of Lung Cancer Cells Caused by Some Anti-cancer Agents (MMC, CPT-11, ADM) is Inhibited by BCL-2

AU - Ohmori, Tohru

AU - Podack, Eckhard R.

AU - Nishio, Kazuto

AU - Takahashi, Minako

AU - Miyahara, Yuki

AU - Takeda, Yuichiro

AU - Kubota, Naohiro

AU - Funayama, Yasunori

AU - Ogasawara, Hayato

AU - Ohira, Tatsuo

AU - Ohta, Sei

AU - Saijo, Nagahiro

PY - 1993

Y1 - 1993

N2 - To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.

AB - To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti- cancer agents by inhibiting the process of apoptosis.

UR - http://www.scopus.com/inward/record.url?scp=0027162207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027162207&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1993.1377

DO - 10.1006/bbrc.1993.1377

M3 - Article

C2 - 8476431

AN - SCOPUS:0027162207

VL - 192

SP - 30

EP - 36

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -