Apoptosis as a form of cell death in intracerebral hemorrhage

Adnan I. Qureshi, M. Fareed K Suri, Peter T. Ostrow, Stanley H. Kim, Zulfiqar Ali, Ahmed A. Shatla, Lee R. Guterman, L. Nelson Hopkins, Ross Bullock, Charles J. Hodge, Roman Hlatky, Alex B. Valadka, James T. Rutka

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

OBJECTIVE: The goals of this study were to identify and quantify the presence of programmed cell death (apoptosis) in intracerebral hemorrhage (ICH) among human subjects. Recent evidence from laboratory models suggests that cell death in the perihematoma region may involve apoptosis. METHODS: Retrospective clinical and histological analyses were performed for patients with spontaneous ICH who underwent surgical evacuation. Quantification of apoptotic cells was performed in sections obtained from the perihematoma region from 12 patients with ICH and stained with the terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick-end labeling method. Necrosis was identified on the basis of morphological criteria, using hematoxylin and eosin staining. RESULTS: Evidence of apoptosis was present in surgical specimens obtained from 10 of the 12 patients. The mean number of apoptotic cells in the perihematoma region in each patient specimen was 38% (range, 0-90%). For five patients, more than one-half of the total cells observed were apoptotic. Apoptosis was observed in specimens obtained within 1 day, 2 days, and 5 days after the onset of symptoms. No terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick-end labeling-positive cells were observed in specimens from the two patients with cerebellar hematomas. The mean proportion of necrotic cells in the perihematoma region in each patient specimen was 25% (range, 0-100%). There was a prominent excess of apoptotic cells, in comparison with necrotic cells, for 6 of the 12 patients who underwent hematoma evacuation. For five other patients, similar proportions of apoptotic and necrotic cells were observed. Necrosis was the predominant finding for only one patient, who underwent late surgical evacuation on Day 5. CONCLUSION: These observations suggest that apoptosis represents a prominent form of cell death associated with ICH in the perihematoma region. Further studies are required to define the mediators of apoptosis in ICH.

Original languageEnglish
Pages (from-to)1041-1048
Number of pages8
JournalNeurosurgery
Volume52
Issue number5
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Cerebral Hemorrhage
Cell Death
Apoptosis
DNA Nucleotidylexotransferase
Hematoma
Necrosis
Hematoxylin
Eosine Yellowish-(YS)
Cell Count
Staining and Labeling

Keywords

  • Apoptosis
  • Hematoxylin and eosin
  • Intracerebral hemorrhage
  • Necrosis
  • Outcome
  • Terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick-end labeling

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Qureshi, A. I., Suri, M. F. K., Ostrow, P. T., Kim, S. H., Ali, Z., Shatla, A. A., ... Rutka, J. T. (2003). Apoptosis as a form of cell death in intracerebral hemorrhage. Neurosurgery, 52(5), 1041-1048.

Apoptosis as a form of cell death in intracerebral hemorrhage. / Qureshi, Adnan I.; Suri, M. Fareed K; Ostrow, Peter T.; Kim, Stanley H.; Ali, Zulfiqar; Shatla, Ahmed A.; Guterman, Lee R.; Hopkins, L. Nelson; Bullock, Ross; Hodge, Charles J.; Hlatky, Roman; Valadka, Alex B.; Rutka, James T.

In: Neurosurgery, Vol. 52, No. 5, 01.05.2003, p. 1041-1048.

Research output: Contribution to journalArticle

Qureshi, AI, Suri, MFK, Ostrow, PT, Kim, SH, Ali, Z, Shatla, AA, Guterman, LR, Hopkins, LN, Bullock, R, Hodge, CJ, Hlatky, R, Valadka, AB & Rutka, JT 2003, 'Apoptosis as a form of cell death in intracerebral hemorrhage', Neurosurgery, vol. 52, no. 5, pp. 1041-1048.
Qureshi AI, Suri MFK, Ostrow PT, Kim SH, Ali Z, Shatla AA et al. Apoptosis as a form of cell death in intracerebral hemorrhage. Neurosurgery. 2003 May 1;52(5):1041-1048.
Qureshi, Adnan I. ; Suri, M. Fareed K ; Ostrow, Peter T. ; Kim, Stanley H. ; Ali, Zulfiqar ; Shatla, Ahmed A. ; Guterman, Lee R. ; Hopkins, L. Nelson ; Bullock, Ross ; Hodge, Charles J. ; Hlatky, Roman ; Valadka, Alex B. ; Rutka, James T. / Apoptosis as a form of cell death in intracerebral hemorrhage. In: Neurosurgery. 2003 ; Vol. 52, No. 5. pp. 1041-1048.
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abstract = "OBJECTIVE: The goals of this study were to identify and quantify the presence of programmed cell death (apoptosis) in intracerebral hemorrhage (ICH) among human subjects. Recent evidence from laboratory models suggests that cell death in the perihematoma region may involve apoptosis. METHODS: Retrospective clinical and histological analyses were performed for patients with spontaneous ICH who underwent surgical evacuation. Quantification of apoptotic cells was performed in sections obtained from the perihematoma region from 12 patients with ICH and stained with the terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick-end labeling method. Necrosis was identified on the basis of morphological criteria, using hematoxylin and eosin staining. RESULTS: Evidence of apoptosis was present in surgical specimens obtained from 10 of the 12 patients. The mean number of apoptotic cells in the perihematoma region in each patient specimen was 38{\%} (range, 0-90{\%}). For five patients, more than one-half of the total cells observed were apoptotic. Apoptosis was observed in specimens obtained within 1 day, 2 days, and 5 days after the onset of symptoms. No terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick-end labeling-positive cells were observed in specimens from the two patients with cerebellar hematomas. The mean proportion of necrotic cells in the perihematoma region in each patient specimen was 25{\%} (range, 0-100{\%}). There was a prominent excess of apoptotic cells, in comparison with necrotic cells, for 6 of the 12 patients who underwent hematoma evacuation. For five other patients, similar proportions of apoptotic and necrotic cells were observed. Necrosis was the predominant finding for only one patient, who underwent late surgical evacuation on Day 5. CONCLUSION: These observations suggest that apoptosis represents a prominent form of cell death associated with ICH in the perihematoma region. Further studies are required to define the mediators of apoptosis in ICH.",
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AU - Hopkins, L. Nelson

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