Apolipoprotein E protects against NMDA excitotoxicity

Mitsuo Aono, Yoonki Lee, Elfrida R. Grant, Robert A. Zivin, Robert D. Pearlstein, David S. Warner, Ellen R. Bennett, Daniel T. Laskowitz

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Preclinical and clinical evidence implicates a role for endogenous apolipoprotein E in modifying the response of the brain to focal and global ischemia. To investigate whether apoE modulates the neuronal response to glutamate excitotoxicity, we exposed primary neuronal glial cultures and a neuronal cell line to biologically relevant concentrations of apolipoprotein E prior to NMDA exposure. In both of these paradigms, apolipoprotein E exerted partial protective effects. At neuroprotective concentrations, however, apolipoprotein E failed to block NMDA-induced calcium influx to the same magnitude as the NMDA receptor antagonist MK-801. These results suggest that one mechanism by which apolipoprotein E modifies the central nervous system response to ischemia may be by reducing glutamate-induced excitotoxicity.

Original languageEnglish
Pages (from-to)214-220
Number of pages7
JournalNeurobiology of Disease
Volume11
Issue number1
DOIs
StatePublished - Dec 19 2002

Fingerprint

Apolipoproteins E
N-Methylaspartate
Glutamic Acid
Ischemia
Dizocilpine Maleate
N-Methyl-D-Aspartate Receptors
Neuroglia
Central Nervous System
Calcium
Cell Line
Brain

Keywords

  • Apolipoprotein E
  • Calcium influx
  • Cell culture
  • Cerebral ischemia
  • Excitotoxicity
  • Neuroprotection
  • NMDA
  • RAP

ASJC Scopus subject areas

  • Neurology

Cite this

Aono, M., Lee, Y., Grant, E. R., Zivin, R. A., Pearlstein, R. D., Warner, D. S., ... Laskowitz, D. T. (2002). Apolipoprotein E protects against NMDA excitotoxicity. Neurobiology of Disease, 11(1), 214-220. https://doi.org/10.1006/nbdi.2002.0541

Apolipoprotein E protects against NMDA excitotoxicity. / Aono, Mitsuo; Lee, Yoonki; Grant, Elfrida R.; Zivin, Robert A.; Pearlstein, Robert D.; Warner, David S.; Bennett, Ellen R.; Laskowitz, Daniel T.

In: Neurobiology of Disease, Vol. 11, No. 1, 19.12.2002, p. 214-220.

Research output: Contribution to journalArticle

Aono, M, Lee, Y, Grant, ER, Zivin, RA, Pearlstein, RD, Warner, DS, Bennett, ER & Laskowitz, DT 2002, 'Apolipoprotein E protects against NMDA excitotoxicity', Neurobiology of Disease, vol. 11, no. 1, pp. 214-220. https://doi.org/10.1006/nbdi.2002.0541
Aono M, Lee Y, Grant ER, Zivin RA, Pearlstein RD, Warner DS et al. Apolipoprotein E protects against NMDA excitotoxicity. Neurobiology of Disease. 2002 Dec 19;11(1):214-220. https://doi.org/10.1006/nbdi.2002.0541
Aono, Mitsuo ; Lee, Yoonki ; Grant, Elfrida R. ; Zivin, Robert A. ; Pearlstein, Robert D. ; Warner, David S. ; Bennett, Ellen R. ; Laskowitz, Daniel T. / Apolipoprotein E protects against NMDA excitotoxicity. In: Neurobiology of Disease. 2002 ; Vol. 11, No. 1. pp. 214-220.
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