ApoA-I induced CD31 in bone marrow-derived vascular progenitor cells increases adhesion: Implications for vascular repair

Karthikeyan Mythreye, Lisa L. Satterwhite, W. Sean Davidson, Pascal J. Goldschmidt-Clermont

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Transgenic over expression of apolipoprotein A-I (ApoA-I) the major structural apolipoprotein of HDL appears to convey the most consistent and strongest anti atherogenic effect observed in animal models so far. We tested the hypothesis that ApoA-I mediates its cardio protective effects additionally through ApoA-I induced differentiation of bone marrow-derived progenitor cells in vitro. This study demonstrates that lineage negative bone marrow cells (lin- BMCs) alter and differentiate in response to free ApoA-I. We find that lin- BMCs in culture treated with recombinant free ApoA-I at a concentration of 0.4 μM are twice as large in size and have altered cell morphology compared to untreated cells; untreated cells retain the original spheroid morphology. Further, the total number of CD31 positive cells in the ApoA-I treated population consistently increased by two fold. This phenotype was significantly reduced in untreated cells and points towards a novel ApoA-I dependent differentiation. A protein lacking its best lipid-binding region (ApoA-IΔ10) did not stimulate any changes in the lin-BMCs indicating that ApoA-I may mediate its effects by regulating cholesterol efflux. The increased CD31 correlates with an increased ability of the lin- BMCs to adhere to both fibronectin and mouse brain endothelial cells. Our results provide the first evidence that exogenous free ApoA-I has the capacity to change the characteristics of progenitor cell populations and suggests a novel mechanism by which HDL may mediate its cardiovascular benefits.

Original languageEnglish (US)
Pages (from-to)703-709
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1781
Issue number11-12
DOIs
StatePublished - Nov 1 2008

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Keywords

  • Adhesion
  • ApoA-I
  • Bone marrow cells (BMCs)
  • CD31
  • Lineage minus
  • Vascular progenitor cell

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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