AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells

Manhui Pang, Ariel F. Martinez, Isabel Fernandez, Wayne E Balkan, Bruce R. Troen

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Cathepsin K (CTSK) is a secreted protease that plays an essential role in osteoclastic bone resorption, and CTSK levels increase with osteoclast differentiation and activation, a process that is controlled by a complex physiological network of hormones and cytokines. A critical regulator of this process is receptor activator of NF-κB ligand (RANKL), a member of the tumor necrosis factor (TNF) superfamily of cytokines that can act via the TNF receptor activating factor (TRAF6)/AP-1 signaling pathway. However, the mechanism whereby RANKL modulates CTSK expression is not fully understood. Therefore, we investigated the regulation of CTSK expression and promoter activity in RAW 264.7 osteoclast precursor cells, which can be readily differentiated to osteoclasts upon RANKL stimulation. Western blot analysis, real-time RT-PCR and luciferase reporter gene assays revealed that RANKL stimulated CTSK expression and promoter activity in a dose- and time-dependent manner and that this activation was inhibited by either dominant negative (DN) TRAF6 or DN-c-fos. TRAF6 stimulated the basal activity of a truncated CTSK promoter, and DN-c-fos blocked this stimulation. JunB alone also stimulated basal CTSK promoter activity, whereas c-jun, JunD or c-fos alone did not. However, co-transfection of any of these jun-family members with c-fos (AP-1) significantly increased CTSK promoter expression. siRNA targeted against c-jun or junB suppressed RANKL-mediated CTSK expression. Therefore, both TRAF6 and AP-1 help regulate the basal and RANKL-mediated stimulation of CTSK gene expression in RAW 264.7 cells.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalGene
Volume403
Issue number1-2
DOIs
StatePublished - Nov 15 2007

Fingerprint

Cathepsin K
Transcription Factor AP-1
TNF Receptor-Associated Factor 6
Osteoclasts
RAW 264.7 Cells
Cytokines
Tumor Necrosis Factor Receptors
Bone Resorption
Luciferases
Reporter Genes
Small Interfering RNA
Transfection
Real-Time Polymerase Chain Reaction
Peptide Hydrolases
Tumor Necrosis Factor-alpha
Western Blotting

Keywords

  • AP-1
  • Cathepsin K
  • Nuclear factor
  • Osteoclast
  • RAW 264.7 cells
  • Transcription

ASJC Scopus subject areas

  • Genetics

Cite this

Pang, M., Martinez, A. F., Fernandez, I., Balkan, W. E., & Troen, B. R. (2007). AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells. Gene, 403(1-2), 151-158. https://doi.org/10.1016/j.gene.2007.08.007

AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells. / Pang, Manhui; Martinez, Ariel F.; Fernandez, Isabel; Balkan, Wayne E; Troen, Bruce R.

In: Gene, Vol. 403, No. 1-2, 15.11.2007, p. 151-158.

Research output: Contribution to journalArticle

Pang, M, Martinez, AF, Fernandez, I, Balkan, WE & Troen, BR 2007, 'AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells', Gene, vol. 403, no. 1-2, pp. 151-158. https://doi.org/10.1016/j.gene.2007.08.007
Pang M, Martinez AF, Fernandez I, Balkan WE, Troen BR. AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells. Gene. 2007 Nov 15;403(1-2):151-158. https://doi.org/10.1016/j.gene.2007.08.007
Pang, Manhui ; Martinez, Ariel F. ; Fernandez, Isabel ; Balkan, Wayne E ; Troen, Bruce R. / AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells. In: Gene. 2007 ; Vol. 403, No. 1-2. pp. 151-158.
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abstract = "Cathepsin K (CTSK) is a secreted protease that plays an essential role in osteoclastic bone resorption, and CTSK levels increase with osteoclast differentiation and activation, a process that is controlled by a complex physiological network of hormones and cytokines. A critical regulator of this process is receptor activator of NF-κB ligand (RANKL), a member of the tumor necrosis factor (TNF) superfamily of cytokines that can act via the TNF receptor activating factor (TRAF6)/AP-1 signaling pathway. However, the mechanism whereby RANKL modulates CTSK expression is not fully understood. Therefore, we investigated the regulation of CTSK expression and promoter activity in RAW 264.7 osteoclast precursor cells, which can be readily differentiated to osteoclasts upon RANKL stimulation. Western blot analysis, real-time RT-PCR and luciferase reporter gene assays revealed that RANKL stimulated CTSK expression and promoter activity in a dose- and time-dependent manner and that this activation was inhibited by either dominant negative (DN) TRAF6 or DN-c-fos. TRAF6 stimulated the basal activity of a truncated CTSK promoter, and DN-c-fos blocked this stimulation. JunB alone also stimulated basal CTSK promoter activity, whereas c-jun, JunD or c-fos alone did not. However, co-transfection of any of these jun-family members with c-fos (AP-1) significantly increased CTSK promoter expression. siRNA targeted against c-jun or junB suppressed RANKL-mediated CTSK expression. Therefore, both TRAF6 and AP-1 help regulate the basal and RANKL-mediated stimulation of CTSK gene expression in RAW 264.7 cells.",
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KW - Cathepsin K

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KW - RAW 264.7 cells

KW - Transcription

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