AP-1 activated by toll-like receptors regulates expression of IL-23 p19

Weicheng Liu, Xinshou Ouyang, Jianjun Yang, Jianguo Liu, Qingshan Li, Yongpeng Gu, Masayuki Fukata, Tony Lin, John Cijiang He, Maria T Abreu, Jay C. Unkeless, Lloyd Meyer, Huabao Xiong

Research output: Contribution to journalArticle

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Abstract

Interleukin (IL)-23, a new member of the IL-12 family, plays a central role in the Th17 immune response and in autoimmune diseases. It is clear that activated macrophages and dendritic cells produce IL-23, but the molecular mechanisms whereby inflammatory signals stimulate IL-23 expression are not fully understood. We demonstrate that induction of IL-23 p19 gene expression by LPS depends on the TLR4 and MyD88 pathways. All three MAPK pathways (ERK, JNK, and p38) that are activated by lipopolysaccharide (LPS) stimulation were shown to exert a positive effect on p19 expression. We cloned a 1.3-kb putative p19 promoter and defined its transcription initiation sites by the 5′-rapid amplification of cDNA ends method. By analyzing IL-23 p19 promoter mutants, we have identified a promoter region (-413 to +10) that contains several important elements, including NF-κB and AP-1. In addition to NF-κB, we have demonstrated that the proximal AP-1 site is important for p19 promoter activation. Mutation of the AP-1 site resulted in the loss of p19 promoter activation. Electrophoretic mobility shift assay (EMSA) analysis showed that c-Jun and c-Fos bind to the AP-1 site, which was confirmed by a chromatin immunoprecipitation assay. Furthermore, co-transfection of c-Jun and ATF2 synergistically induced p19 promoter activation, and c-Jun and ATF2 formed a protein complex, demonstrated by co-immunoprecipitation. Finally, LPS-stimulated peritoneal macrophages from IL-10-deficient mice expressed significantly higher IL-23 p19 than macrophages from wild type mice, and the addition of recombinant IL-10 strongly inhibited LPS-induced p19 expression. Thus, this study suggests that MyD88-dependent Toll-like receptor signaling induces IL-23 p19 gene expression through both MAPKs and NF-κB.

Original languageEnglish
Pages (from-to)24006-24016
Number of pages11
JournalJournal of Biological Chemistry
Volume284
Issue number36
DOIs
StatePublished - Sep 4 2009
Externally publishedYes

Fingerprint

Interleukin-23
Toll-Like Receptors
Transcription Factor AP-1
Lipopolysaccharides
Macrophages
MAP Kinase Signaling System
Chemical activation
Gene expression
Interleukin-10
Assays
Gene Expression
Electrophoretic mobility
Chromatin Immunoprecipitation
Transcription Initiation Site
Peritoneal Macrophages
Electrophoretic Mobility Shift Assay
Interleukin-12
Immunoprecipitation
Genetic Promoter Regions
Dendritic Cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Liu, W., Ouyang, X., Yang, J., Liu, J., Li, Q., Gu, Y., ... Xiong, H. (2009). AP-1 activated by toll-like receptors regulates expression of IL-23 p19. Journal of Biological Chemistry, 284(36), 24006-24016. https://doi.org/10.1074/jbc.M109.025528

AP-1 activated by toll-like receptors regulates expression of IL-23 p19. / Liu, Weicheng; Ouyang, Xinshou; Yang, Jianjun; Liu, Jianguo; Li, Qingshan; Gu, Yongpeng; Fukata, Masayuki; Lin, Tony; He, John Cijiang; Abreu, Maria T; Unkeless, Jay C.; Meyer, Lloyd; Xiong, Huabao.

In: Journal of Biological Chemistry, Vol. 284, No. 36, 04.09.2009, p. 24006-24016.

Research output: Contribution to journalArticle

Liu, W, Ouyang, X, Yang, J, Liu, J, Li, Q, Gu, Y, Fukata, M, Lin, T, He, JC, Abreu, MT, Unkeless, JC, Meyer, L & Xiong, H 2009, 'AP-1 activated by toll-like receptors regulates expression of IL-23 p19', Journal of Biological Chemistry, vol. 284, no. 36, pp. 24006-24016. https://doi.org/10.1074/jbc.M109.025528
Liu, Weicheng ; Ouyang, Xinshou ; Yang, Jianjun ; Liu, Jianguo ; Li, Qingshan ; Gu, Yongpeng ; Fukata, Masayuki ; Lin, Tony ; He, John Cijiang ; Abreu, Maria T ; Unkeless, Jay C. ; Meyer, Lloyd ; Xiong, Huabao. / AP-1 activated by toll-like receptors regulates expression of IL-23 p19. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 36. pp. 24006-24016.
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AU - Gu, Yongpeng

AU - Fukata, Masayuki

AU - Lin, Tony

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