Antiviral potency of a siRNA targeting a conserved region of coxsackievirus A24

Eun Jung Jun, Young Ran Nam, Jeonghyun Ahn, Hungwon Tchah, Chul Hyun Joo, Youngmee Jee, Yoo Kyum Kim, Heuiran Lee

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Coxsackievirus A24 (CVA24) is responsible for acute hemorrhagic conjunctivitis, a highly contagious eye disease for which no prevention or treatment is currently available. We thus assessed the antiviral potential of a small interfering RNA (siRNA) targeting CVA24. HeLa cells with or without four different siRNAs complementary to 2C or 3D genome region, were challenged with various CVA24s. Among several siRNAs, a siRNA targeting the highly conserved genome region called the cis-acting replication element (CVA24-CRE), was the only siRNA that decreased virus replication and subsequent cytotoxicity by both CVA24 variant and clinical isolates. Furthermore, CVA24-CRE had effective antiviral activity against CVA24 in primary human conjunctival cells. In addition, CVA24-CRE was highly resistant to the emergence of genetically altered escape mutants. Collectively, the present study provides evidence that CVA24-CRE targeting a conserved viral genome region had universal, prolonged anti-CVA24 activity. This siRNA may thus hold a potential to act clinically as a novel anti-CVA24 agent.

Original languageEnglish (US)
Pages (from-to)389-394
Number of pages6
JournalBiochemical and biophysical research communications
Volume376
Issue number2
DOIs
StatePublished - Nov 14 2008

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Keywords

  • Acute hemorrhagic conjunctivitis
  • Antiviral effect
  • Coxsackievirus A24
  • Small-interfering RNA
  • cis-Acting replication element

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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