Antioxidant enzymes reduce loss of blood-brain barrier integrity in experimental optic neuritis

John Guy, E. A. Ellis, G. M. Hope, N. A. Rao

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We studied the effect of antioxidant enzymes on the loss of integrity of the blood-brain barrier in the optic nerves of strain-13 guinea pigs with chronic experimental allergic encephalomyelitis, a demyelinating disorder with neurologic and histopathologic characteristics similar to multiple sclerosis. Animals with experimental allergic encephalomyelitis received daily intraperitoneal injections of either preservative-free saline (group 1), catalase (group 2), or glutathione peroxidase (group 3) for 2.2 months after the onset of appendicular paralysis. Following intravascular administration, extravascular leakage of horseradish peroxidase was histopathologically graded as mild, moderate, or severe within the optic nerve head and myelinated retrolaminar nerve. Severe extravasation of horseradish peroxidase was exclusive to group 1, in addition to moderate and mild leakage. In groups 2 and 3, leakage of horseradish peroxidase was infrequent, and when detected, it was graded as mild. Detoxification of hydrogen peroxide with catalase and glutathione peroxidase substantially reduced horseradish peroxidase leakage in experimental optic neuritis, suggesting a role for hydrogen peroxide and its reactive by-products in the pathogenesis of increased vascular permeability of the blood-brain barrier in experimental allergic encephalomyelitis.

Original languageEnglish
Pages (from-to)1359-1363
Number of pages5
JournalArchives of Ophthalmology
Volume107
Issue number9
StatePublished - Jan 1 1989
Externally publishedYes

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Optic Neuritis
Horseradish Peroxidase
Blood-Brain Barrier
Autoimmune Experimental Encephalomyelitis
Antioxidants
Enzymes
Glutathione Peroxidase
Catalase
Hydrogen Peroxide
Optic Disk
Capillary Permeability
Demyelinating Diseases
Optic Nerve
Intraperitoneal Injections
Paralysis
Nervous System
Multiple Sclerosis
Guinea Pigs

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Antioxidant enzymes reduce loss of blood-brain barrier integrity in experimental optic neuritis. / Guy, John; Ellis, E. A.; Hope, G. M.; Rao, N. A.

In: Archives of Ophthalmology, Vol. 107, No. 9, 01.01.1989, p. 1359-1363.

Research output: Contribution to journalArticle

Guy, John ; Ellis, E. A. ; Hope, G. M. ; Rao, N. A. / Antioxidant enzymes reduce loss of blood-brain barrier integrity in experimental optic neuritis. In: Archives of Ophthalmology. 1989 ; Vol. 107, No. 9. pp. 1359-1363.
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