Antioxidant and electron donating function of hypothalamic polypeptides: Galarmin and Gx-NH2

L. A. Tavadyan, K. A. Galoian, L. A. Harutunyan, H. G. Tonikyan, A. A. Galoyan

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Chemical mechanisms of antioxidant and electron donating function of the hypothalamic proline-rich polypeptides have been clarified on the molecular level. The antioxidant-chelating property of Galarmin and Gx-NH2 was established by their capability to inhibit copper(II) dichloride catalyzed H2O2 decomposition, thus preventing formation of HO and HOO radicals. The antiradical activity of Galarmin and Gx-NH2 was determined by their ability to react with 2,2-diphenyl-1-picrylhydrazyl radical applying differential pulse voltammetry and UV-Vis spectrophotometry methods. Galarmin manifest antiradical activity towards 2,2-diphenyl-1-picrylhydrazyl radical, depending on the existence of phenolic OH group in tyrosine residue at the end of the molecule. The presence of antiradical activity and reduction properties of Galarmin are confirmed by the existence of an oxidation specific peak in voltammograms made by differential pulse voltammetry at E ̊ = 0.795 V vs. Ag/Ag + aq.

Original languageEnglish (US)
Pages (from-to)947-952
Number of pages6
JournalNeurochemical Research
Volume35
Issue number6
DOIs
StatePublished - Jun 1 2010

Keywords

  • Antioxidant
  • Antiradical activity
  • Chelator
  • DPV
  • Electron donator
  • Proline-rich peptide

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry

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