Antimycotic Efficacy and Safety of a New Cold Corneal Storage Medium by Time-Kill and Toxicity Studies

Laura Giurgola, Claudio Gatto, Jean-Marie A Parel, Darlene Miller, Jana DʼAmato Tóthová

Research output: Contribution to journalArticle

Abstract

PURPOSE: To evaluate a new corneal cold storage medium including an antimycotic tablet (Kerasave, AL.CHI.MI.A. S.r.l.). METHODS: Kerasave and tryptone soy broth (control) were inoculated with 10 and 10 colony-forming units (CFU)/mL of 6 Candida isolates (Candida albicans [n = 4], Candida tropicalis [n = 1], and Candida glabrata [n = 1]). Minimum inhibitory concentrations (MICs) were determined using amphotericin B Etest strips. Sterile porcine corneas contaminated with 10 CFU/mL of each isolate were incubated in Kerasave and control at 4°C. Growth rate and Log10 reduction at 4°C at different time intervals were determined for liquid samples and tissue homogenates. Kerasave biocompatibility was assessed according to ISO 10993-5 and ISO 10993-10. RESULTS: No C. albicans or C. tropicalis colonies were recovered from Kerasave inoculated with 10 CFU/mL after incubation for 3 days at 4°C. C. glabrata was inhibited but not killed after 3 days at 4°C. Four of the 6 strains contaminated with 10 CFU/mL demonstrated a significant ≥ 3 Log10 reduction in media and tissue homogenates within 5 days as compared to controls (p < 0.01). Amphotericin B MICs ranged from 0.19 to 0.38 μg/mL for C. albicans (n = 3) and C. tropicalis (n = 1). C. glabrata showed reduced susceptibility (0.5 μg/mL) and 1 C. albicans was resistant to amphotericin B (≥ 1 μg/mL). Kerasave was not cytotoxic, irritating, or sensitizing according to the ISO standards. CONCLUSIONS: Kerasave showed high antifungal efficacy against susceptible fungal strains at 4°C in the presence and absence of corneal tissue. Resistant strains to amphotericin B were not eliminated by Kerasave. Kerasave is not cytotoxic, irritating, or sensitizing.

Original languageEnglish (US)
Pages (from-to)1314-1321
Number of pages8
JournalCornea
Volume38
Issue number10
DOIs
StatePublished - Oct 1 2019

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Amphotericin B
Candida tropicalis
Candida albicans
Candida glabrata
Stem Cells
Safety
Microbial Sensitivity Tests
Disk Diffusion Antimicrobial Tests
Candida
Cornea
Tablets
Swine
Growth

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Antimycotic Efficacy and Safety of a New Cold Corneal Storage Medium by Time-Kill and Toxicity Studies. / Giurgola, Laura; Gatto, Claudio; Parel, Jean-Marie A; Miller, Darlene; DʼAmato Tóthová, Jana.

In: Cornea, Vol. 38, No. 10, 01.10.2019, p. 1314-1321.

Research output: Contribution to journalArticle

Giurgola, Laura ; Gatto, Claudio ; Parel, Jean-Marie A ; Miller, Darlene ; DʼAmato Tóthová, Jana. / Antimycotic Efficacy and Safety of a New Cold Corneal Storage Medium by Time-Kill and Toxicity Studies. In: Cornea. 2019 ; Vol. 38, No. 10. pp. 1314-1321.
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