Antimicrobial activity of UMFix tissue fixative

Timothy Cleary, Azorides R Morales, Mehrdad Nadji, M. Nassiri, V. Vincek

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aims: The aim of this study was to determine the antimicrobial effects of UMFix, an alcohol based tissue fixative, on various microorganisms. The UMFix solution was compared with 10% neutral buffered formalin. Methods: Standard methods to determine microorganism colony counts were performed after exposure of the microorganisms to UMFix and 10% neutral buffered formalin. Results: After a short exposure, UMFix rapidly killed vegetative bacteria, yeasts, moulds, and viruses. Bacterial spores were resistant to killing by UMFix. All organisms were killed by the 10% neutral buffered formalin preparation. Conclusions: UMFix was microbicidal for vegetative bacteria, yeasts, and aspergillus species after a short exposure, although it was not active against spore forming bacillus species. The methanol content of the fixative was responsible for the killing effect of this fixative. No killing was seen when polyethylene glycol was used alone.

Original languageEnglish
Pages (from-to)22-25
Number of pages4
JournalJournal of Clinical Pathology
Volume58
Issue number1
DOIs
StatePublished - Jan 1 2005

Fingerprint

Fixatives
Formaldehyde
Yeasts
Bacterial Spores
Bacteria
Aspergillus
Spores
Bacillus
Methanol
Fungi
Alcohols
Viruses

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Antimicrobial activity of UMFix tissue fixative. / Cleary, Timothy; Morales, Azorides R; Nadji, Mehrdad; Nassiri, M.; Vincek, V.

In: Journal of Clinical Pathology, Vol. 58, No. 1, 01.01.2005, p. 22-25.

Research output: Contribution to journalArticle

Cleary, T, Morales, AR, Nadji, M, Nassiri, M & Vincek, V 2005, 'Antimicrobial activity of UMFix tissue fixative', Journal of Clinical Pathology, vol. 58, no. 1, pp. 22-25. https://doi.org/10.1136/jcp.2004.021618
Cleary, Timothy ; Morales, Azorides R ; Nadji, Mehrdad ; Nassiri, M. ; Vincek, V. / Antimicrobial activity of UMFix tissue fixative. In: Journal of Clinical Pathology. 2005 ; Vol. 58, No. 1. pp. 22-25.
@article{3e0903a5499c4db8a4fb135262f88fe6,
title = "Antimicrobial activity of UMFix tissue fixative",
abstract = "Aims: The aim of this study was to determine the antimicrobial effects of UMFix, an alcohol based tissue fixative, on various microorganisms. The UMFix solution was compared with 10{\%} neutral buffered formalin. Methods: Standard methods to determine microorganism colony counts were performed after exposure of the microorganisms to UMFix and 10{\%} neutral buffered formalin. Results: After a short exposure, UMFix rapidly killed vegetative bacteria, yeasts, moulds, and viruses. Bacterial spores were resistant to killing by UMFix. All organisms were killed by the 10{\%} neutral buffered formalin preparation. Conclusions: UMFix was microbicidal for vegetative bacteria, yeasts, and aspergillus species after a short exposure, although it was not active against spore forming bacillus species. The methanol content of the fixative was responsible for the killing effect of this fixative. No killing was seen when polyethylene glycol was used alone.",
author = "Timothy Cleary and Morales, {Azorides R} and Mehrdad Nadji and M. Nassiri and V. Vincek",
year = "2005",
month = "1",
day = "1",
doi = "10.1136/jcp.2004.021618",
language = "English",
volume = "58",
pages = "22--25",
journal = "Journal of Clinical Pathology - Clinical Molecular Pathology",
issn = "0021-9746",
publisher = "BMJ Publishing Group",
number = "1",

}

TY - JOUR

T1 - Antimicrobial activity of UMFix tissue fixative

AU - Cleary, Timothy

AU - Morales, Azorides R

AU - Nadji, Mehrdad

AU - Nassiri, M.

AU - Vincek, V.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Aims: The aim of this study was to determine the antimicrobial effects of UMFix, an alcohol based tissue fixative, on various microorganisms. The UMFix solution was compared with 10% neutral buffered formalin. Methods: Standard methods to determine microorganism colony counts were performed after exposure of the microorganisms to UMFix and 10% neutral buffered formalin. Results: After a short exposure, UMFix rapidly killed vegetative bacteria, yeasts, moulds, and viruses. Bacterial spores were resistant to killing by UMFix. All organisms were killed by the 10% neutral buffered formalin preparation. Conclusions: UMFix was microbicidal for vegetative bacteria, yeasts, and aspergillus species after a short exposure, although it was not active against spore forming bacillus species. The methanol content of the fixative was responsible for the killing effect of this fixative. No killing was seen when polyethylene glycol was used alone.

AB - Aims: The aim of this study was to determine the antimicrobial effects of UMFix, an alcohol based tissue fixative, on various microorganisms. The UMFix solution was compared with 10% neutral buffered formalin. Methods: Standard methods to determine microorganism colony counts were performed after exposure of the microorganisms to UMFix and 10% neutral buffered formalin. Results: After a short exposure, UMFix rapidly killed vegetative bacteria, yeasts, moulds, and viruses. Bacterial spores were resistant to killing by UMFix. All organisms were killed by the 10% neutral buffered formalin preparation. Conclusions: UMFix was microbicidal for vegetative bacteria, yeasts, and aspergillus species after a short exposure, although it was not active against spore forming bacillus species. The methanol content of the fixative was responsible for the killing effect of this fixative. No killing was seen when polyethylene glycol was used alone.

UR - http://www.scopus.com/inward/record.url?scp=11244305912&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11244305912&partnerID=8YFLogxK

U2 - 10.1136/jcp.2004.021618

DO - 10.1136/jcp.2004.021618

M3 - Article

C2 - 15623477

AN - SCOPUS:11244305912

VL - 58

SP - 22

EP - 25

JO - Journal of Clinical Pathology - Clinical Molecular Pathology

JF - Journal of Clinical Pathology - Clinical Molecular Pathology

SN - 0021-9746

IS - 1

ER -