Antigenic analysis of hematopoiesis. V. Characterization of My-10 antigen expression by normal lymphohematopoietic progenitor cells

L. C. Strauss, S. D. Rowley, V. F. la Russa, S. J. Sharkis, R. K. Stuart, C. I. Civin

Research output: Contribution to journalArticle

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Abstract

The My-10 glycoprotein is an hematopoietic cell surface antigen expressed specifically by undifferentiated (blast) cells, constituting 1%-4% of normal adult bone marrow leukocytes. We used several immunological and in vitro culture methods to analyze the expression of this unique antigen on a variety of lymphohematopoietic progenitor cells. Colony-forming cells (CFC) for granulocyte-monocyte colonies (CFC-GM) and erythroid colonies (BFU-E) were predominantly My-10 positive. CFC with higher proliferative potential were more strongly My-10 positive than CFC with lower proliferative potential, and those for mixed-lineage and blast cell colonies were even more uniformly My-10 positive. Cells maintaining CFC-GM number in short-term marrow culture (pre-CFC) were found to be My-10 positive, as were lymphoid precursors defined by their content of intranuclear terminal deoxynucleotidyl transferase. More mature erythroid precursors (CFU-E) were heterogeneous for antigen expression and lost My-10 antigen progressively, in parallel with advancing maturational stage. The My-10 antigen permits rapid identification and purification of hematopoietic progenitor cells for further study or potential clinical application. The disappearance of the My-10 antigen, moreover, may be a probe for differentiation-linked cellular events.

Original languageEnglish
Pages (from-to)878-886
Number of pages9
JournalExperimental Hematology
Volume14
Issue number9
StatePublished - Dec 1 1986

Fingerprint

Hematopoiesis
Stem Cells
Erythroid Precursor Cells
Bone Marrow
Antigens
DNA Nucleotidylexotransferase
Surface Antigens
Hematopoietic Stem Cells
My-10 glycoprotein
Granulocytes
Monocytes
Leukocytes
Cell Count

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Strauss, L. C., Rowley, S. D., la Russa, V. F., Sharkis, S. J., Stuart, R. K., & Civin, C. I. (1986). Antigenic analysis of hematopoiesis. V. Characterization of My-10 antigen expression by normal lymphohematopoietic progenitor cells. Experimental Hematology, 14(9), 878-886.

Antigenic analysis of hematopoiesis. V. Characterization of My-10 antigen expression by normal lymphohematopoietic progenitor cells. / Strauss, L. C.; Rowley, S. D.; la Russa, V. F.; Sharkis, S. J.; Stuart, R. K.; Civin, C. I.

In: Experimental Hematology, Vol. 14, No. 9, 01.12.1986, p. 878-886.

Research output: Contribution to journalArticle

Strauss, LC, Rowley, SD, la Russa, VF, Sharkis, SJ, Stuart, RK & Civin, CI 1986, 'Antigenic analysis of hematopoiesis. V. Characterization of My-10 antigen expression by normal lymphohematopoietic progenitor cells', Experimental Hematology, vol. 14, no. 9, pp. 878-886.
Strauss, L. C. ; Rowley, S. D. ; la Russa, V. F. ; Sharkis, S. J. ; Stuart, R. K. ; Civin, C. I. / Antigenic analysis of hematopoiesis. V. Characterization of My-10 antigen expression by normal lymphohematopoietic progenitor cells. In: Experimental Hematology. 1986 ; Vol. 14, No. 9. pp. 878-886.
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