Antigen stimulates glycoprotein secretion and alters ion fluxes in sheep trachea

R. J. Phipps, S. M. Denas, Adam Wanner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We studied the effects of in vitro challenge with specific antigen (Ascaris suum antigen) on glycoprotein secretion and ion fluxes in tracheal tissues from allergic sheep. We mounted tissues in Perspex chambers and measured secretion of 35S- and 3H-labeled glycoproteins and fluxes of Cl- and Na+. In tissues from allergic sheep, A. suum antigen (25 μg protein·ml-1) increased glycoprotein secretion. A. suum antigen initially reversed net Cl- flux, causing net absorption of Cl- and Na+. This was followed 15-30 min later by net secretion of Cl- and of Na+. Pretreatment of tissues with cromolyn (10-4 M) greatly reduced the effects of A. suum antigen but did not abolish them. The cromolyn-resistant effects were nonspecific, because they were similar to those of in vitro challenges with nonspecific proteins, ovalbumin and ragweed in allergic sheep, and A. suum antigen in nonallergic sheep. We conclude that challenge with A suum antigen results in mucus hypersecretion in airways of allergic sheep, by both specific and smaller nonspecific effects. Specific effects (cromolyn sensitive) are produced by mediators which are released from airway cells in response to A. suum challenge.

Original languageEnglish
Pages (from-to)1593-1602
Number of pages10
JournalJournal of Applied Physiology Respiratory Environmental and Exercise Physiology
Volume55
Issue number5
StatePublished - Dec 1 1983

Fingerprint

Ascaris suum
Trachea
Sheep
Glycoproteins
Ions
Antigens
Cromolyn Sodium
Ambrosia
Ovalbumin
Polymethyl Methacrylate
Mucus

ASJC Scopus subject areas

  • Endocrinology
  • Physiology

Cite this

Antigen stimulates glycoprotein secretion and alters ion fluxes in sheep trachea. / Phipps, R. J.; Denas, S. M.; Wanner, Adam.

In: Journal of Applied Physiology Respiratory Environmental and Exercise Physiology, Vol. 55, No. 5, 01.12.1983, p. 1593-1602.

Research output: Contribution to journalArticle

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