Antigen retrieval using ph 3.5 glycine-HCI buffer or urea solution for immunohistochemical localization of ki-67

Shan Rong Shi, Benjaporn Chaiwun, Lillian Young, Ashraf Imam, Richard J. Cote, Clive R. Taylor

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A new antibody (MIB-1) has been described, permitting the demonstration of Ki-67 proliferation antigen in paraffin sections. However, satisfactory results were obtained only after subjecting tissue sections to microwave based antigen retrieval in citrate buffer solution. Other buffer solutions produce equivalent or better results and also permit use of the original Ki-67 antibody, which hitherto has been considered ineffective for paraffin sections.

Original languageEnglish (US)
Pages (from-to)213-215
Number of pages3
JournalBiotechnic and Histochemistry
Volume69
Issue number4
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

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