Antigen retrieval immunohistochemistry used for routinely processed celloidin-embedded human temporal bone sections: Standardization and development

Shan Rong Shi, Richard J. Cote, Clive R. Taylor

Research output: Contribution to journalReview articlepeer-review

16 Scopus citations

Abstract

The use of immunohistochemistry (IHC) in routinely processed celloidin- embedded human temporal bone section has created a fruitful field in understanding the pathogenesis and pathophysiology of the human inner ear at a molecular level since the early 1990s when the antigen retrieval (AR) technique was developed. This review article focuses on several critical technical issues of AR technique based predominantly on our experiences and suggestions concerning further development and standardization of AR-IHC for IHC study of human temporal bone section, as well as other tissues embedded in celloidin. Examples of using AR-IHC in human temporal bone sections collected include our unpublished data in order to indicate the potential utility of this novel method. Suggestions of further development of AR techniques are proposed for references and comments.

Original languageEnglish (US)
Pages (from-to)425-443
Number of pages19
JournalAuris Nasus Larynx
Volume25
Issue number4
DOIs
StatePublished - Dec 1 1998
Externally publishedYes

Keywords

  • Antigen retrieval
  • Celloidin-embedding tissue
  • Human temporal bone
  • Immunohistochemistry
  • Microwave
  • Otopathology

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Surgery

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