Antigen retrieval for immunohistochemistry status and need for greater standardization

Clive R. Taylor, Shan Rong Shi, Richard J Cote

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Antigen retrieval (AR) describes a high-temperature unmasking method as well as a nonheating method that is utilized prior to immunohistochemical staining of fixed tissue sections with the goal of improving the quality of the resulting stain. The high-temperature method has been applied extensively to formalin-fixed paraffin-embedded tissue sections, the materials most commonly available to the surgical pathologist. Following the introduction of AR in 1991, there have been numerous articles demonstrating the value of this method for routine immunohistochemistry. More than 200 antibodies have been tested, with excellent immunohistochemical staining in almost all instances. The principles of the AR technique are reviewed, and emphasis is placed on standardization and current application.

Original languageEnglish
Pages (from-to)144-166
Number of pages23
JournalApplied Immunohistochemistry and Molecular Morphology
Volume4
Issue number3
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Immunohistochemistry
Antigens
Staining and Labeling
Temperature
Paraffin
Formaldehyde
Coloring Agents
Antibodies

Keywords

  • Antibody
  • Antigen
  • Antigen retrieval
  • Formalin
  • Heat-induced epitope retrieval
  • Immunohistochemistry
  • Paraffin tissue sections

ASJC Scopus subject areas

  • Anatomy
  • Medical Laboratory Technology

Cite this

Antigen retrieval for immunohistochemistry status and need for greater standardization. / Taylor, Clive R.; Shi, Shan Rong; Cote, Richard J.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 4, No. 3, 01.12.1996, p. 144-166.

Research output: Contribution to journalArticle

@article{0ac1baee02dd4cbca645dd7fcf78939f,
title = "Antigen retrieval for immunohistochemistry status and need for greater standardization",
abstract = "Antigen retrieval (AR) describes a high-temperature unmasking method as well as a nonheating method that is utilized prior to immunohistochemical staining of fixed tissue sections with the goal of improving the quality of the resulting stain. The high-temperature method has been applied extensively to formalin-fixed paraffin-embedded tissue sections, the materials most commonly available to the surgical pathologist. Following the introduction of AR in 1991, there have been numerous articles demonstrating the value of this method for routine immunohistochemistry. More than 200 antibodies have been tested, with excellent immunohistochemical staining in almost all instances. The principles of the AR technique are reviewed, and emphasis is placed on standardization and current application.",
keywords = "Antibody, Antigen, Antigen retrieval, Formalin, Heat-induced epitope retrieval, Immunohistochemistry, Paraffin tissue sections",
author = "Taylor, {Clive R.} and Shi, {Shan Rong} and Cote, {Richard J}",
year = "1996",
month = "12",
day = "1",
language = "English",
volume = "4",
pages = "144--166",
journal = "Applied Immunohistochemistry and Molecular Morphology",
issn = "1541-2016",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Antigen retrieval for immunohistochemistry status and need for greater standardization

AU - Taylor, Clive R.

AU - Shi, Shan Rong

AU - Cote, Richard J

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Antigen retrieval (AR) describes a high-temperature unmasking method as well as a nonheating method that is utilized prior to immunohistochemical staining of fixed tissue sections with the goal of improving the quality of the resulting stain. The high-temperature method has been applied extensively to formalin-fixed paraffin-embedded tissue sections, the materials most commonly available to the surgical pathologist. Following the introduction of AR in 1991, there have been numerous articles demonstrating the value of this method for routine immunohistochemistry. More than 200 antibodies have been tested, with excellent immunohistochemical staining in almost all instances. The principles of the AR technique are reviewed, and emphasis is placed on standardization and current application.

AB - Antigen retrieval (AR) describes a high-temperature unmasking method as well as a nonheating method that is utilized prior to immunohistochemical staining of fixed tissue sections with the goal of improving the quality of the resulting stain. The high-temperature method has been applied extensively to formalin-fixed paraffin-embedded tissue sections, the materials most commonly available to the surgical pathologist. Following the introduction of AR in 1991, there have been numerous articles demonstrating the value of this method for routine immunohistochemistry. More than 200 antibodies have been tested, with excellent immunohistochemical staining in almost all instances. The principles of the AR technique are reviewed, and emphasis is placed on standardization and current application.

KW - Antibody

KW - Antigen

KW - Antigen retrieval

KW - Formalin

KW - Heat-induced epitope retrieval

KW - Immunohistochemistry

KW - Paraffin tissue sections

UR - http://www.scopus.com/inward/record.url?scp=0029796770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029796770&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0029796770

VL - 4

SP - 144

EP - 166

JO - Applied Immunohistochemistry and Molecular Morphology

JF - Applied Immunohistochemistry and Molecular Morphology

SN - 1541-2016

IS - 3

ER -