Abstract
Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 667-672 |
| Number of pages | 6 |
| Journal | Analyst |
| Volume | 133 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 2008 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry
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Triulzi, R. C., Micic, M., Orbulescu, J., Giordani, S., Mueller, B., & Leblanc, R. M. (2008). Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay. Analyst, 133(5), 667-672. https://doi.org/10.1039/b718730k