Abstract
Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 667-672 |
| Number of pages | 6 |
| Journal | Analyst |
| Volume | 133 |
| Issue number | 5 |
| DOIs | |
| State | Published - Apr 28 2008 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay. / Triulzi, Robert C.; Micic, Miodrag; Orbulescu, Jhony; Giordani, Silvia; Mueller, Bill; Leblanc, Roger.
In: Analyst, Vol. 133, No. 5, 28.04.2008, p. 667-672.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay
AU - Triulzi, Robert C.
AU - Micic, Miodrag
AU - Orbulescu, Jhony
AU - Giordani, Silvia
AU - Mueller, Bill
AU - Leblanc, Roger
PY - 2008/4/28
Y1 - 2008/4/28
N2 - Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.
AB - Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.
UR - http://www.scopus.com/inward/record.url?scp=42449095064&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42449095064&partnerID=8YFLogxK
U2 - 10.1039/b718730k
DO - 10.1039/b718730k
M3 - Article
C2 - 18427690
AN - SCOPUS:42449095064
VL - 133
SP - 667
EP - 672
JO - The Analyst
JF - The Analyst
SN - 0003-2654
IS - 5
ER -