Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay

Robert C. Triulzi, Miodrag Micic, Jhony Orbulescu, Silvia Giordani, Bill Mueller, Roger M. Leblanc

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.

Original languageEnglish (US)
Pages (from-to)667-672
Number of pages6
JournalAnalyst
Volume133
Issue number5
DOIs
StatePublished - Jan 1 2008

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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    Triulzi, R. C., Micic, M., Orbulescu, J., Giordani, S., Mueller, B., & Leblanc, R. M. (2008). Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay. Analyst, 133(5), 667-672. https://doi.org/10.1039/b718730k