Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay

Robert C. Triulzi, Miodrag Micic, Jhony Orbulescu, Silvia Giordani, Bill Mueller, Roger M. Leblanc

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.

Original languageEnglish (US)
Pages (from-to)667-672
Number of pages6
Issue number5
StatePublished - Jan 1 2008

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry


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