Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay

Robert C. Triulzi, Miodrag Micic, Jhony Orbulescu, Silvia Giordani, Bill Mueller, Roger Leblanc

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.

Original languageEnglish
Pages (from-to)667-672
Number of pages6
JournalAnalyst
Volume133
Issue number5
DOIs
StatePublished - Apr 28 2008

Fingerprint

Quantum Dots
Dendrimers
immunoassay
Immunoassay
Antibodies
Gold
Semiconductor quantum dots
antibody
Immunoglobulins
Quenching
gold
Immunoglobulin G
goat
fluorescence
Goats
Fluorescence
PAMAM Starburst

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Triulzi, R. C., Micic, M., Orbulescu, J., Giordani, S., Mueller, B., & Leblanc, R. (2008). Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay. Analyst, 133(5), 667-672. https://doi.org/10.1039/b718730k

Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay. / Triulzi, Robert C.; Micic, Miodrag; Orbulescu, Jhony; Giordani, Silvia; Mueller, Bill; Leblanc, Roger.

In: Analyst, Vol. 133, No. 5, 28.04.2008, p. 667-672.

Research output: Contribution to journalArticle

Triulzi, RC, Micic, M, Orbulescu, J, Giordani, S, Mueller, B & Leblanc, R 2008, 'Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay', Analyst, vol. 133, no. 5, pp. 667-672. https://doi.org/10.1039/b718730k
Triulzi RC, Micic M, Orbulescu J, Giordani S, Mueller B, Leblanc R. Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay. Analyst. 2008 Apr 28;133(5):667-672. https://doi.org/10.1039/b718730k
Triulzi, Robert C. ; Micic, Miodrag ; Orbulescu, Jhony ; Giordani, Silvia ; Mueller, Bill ; Leblanc, Roger. / Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay. In: Analyst. 2008 ; Vol. 133, No. 5. pp. 667-672.
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