Antibodies Against the Bovine Brain Glutamate Binding Protein

Sabita Roy, Elias K. Michaelis

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Abstract: Antibodies against the purified bovine brain glutamate binding protein (GBP) were raised in rabbits. Both nonderivatized and dinitrobenzene‐derivatized GBP produced strong immunological responses in rabbits. Using the enzyme‐linked immunosorbent assay (ELISA), we have quantified the antibody production and determined the specificity of the antibodies. Bovine brain GBP and the analogous protein from rat brain interacted most strongly with the antibodies. A bacterial glutamate‐aspartate binding protein, as well as the enzymes glutamate dehydrogenase (EC 1.4.1.3), glutamine synthetase (EC 6.3.1.2), and γ‐glutamyl transpeptidase (EC 2.3.2.2), showed little or no cross‐reactivity with the anti‐GBP antibodies. A crude bacterial glutamate decarboxylase (EC 4.1.1.15) preparation gave a small to moderate cross‐reaction with the anti‐GBP antibodies. The sensitivity of the ELISA assay and the specificity of the antibodies were such that GBP levels as low as 3–10 ng could be detected.

Original languageEnglish (US)
Pages (from-to)838-841
Number of pages4
JournalJournal of neurochemistry
Volume42
Issue number3
DOIs
StatePublished - Mar 1984

Keywords

  • Antibodies
  • Binding protein
  • Bovine brain
  • ELISA
  • Glutamate enzymes
  • L‐Glutamate
  • Rat brain

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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