Antibacterial effectiveness of commercial eyedrop preservatives

K. B. Kirn, I. Barequet, T. K. Chan, G. J. Osterhout, Terrence O'Brien

Research output: Contribution to journalArticle

Abstract

Purpose: To test the sustained and immediate antibacterial effectiveness of several preservative systems in commonly used multiple-dose eyedrops. Methods: Standardized suspensions of each of 4 test organisms (P. aeruginosa, S. marcesens, S. aureus, S. epidermidis) were added daily to commercial solutions containing thimerosal (Fluoracaine), benzalkonium chloride (Tropicamide 1% and Alcaine) and polyaminopropyl biguanide (ReNu Multipurpose CL solution). Serial samples were obtained and quantitative bacteriology was performed weekly. Preservative systems were also acutely challenged with high bacterial concentration ( 1 x 108 or Ix 1018 cfu/ml ) and sampled for immediate plating. Results; Serial cultures failed to recover any growth of test organisms for up to 8 weeks of testing. Benzalkonium chloride was observed to have a stronger immediate antibacterial effect with higher inoculum concentrations as compared with the other two preservatives. Conclusion: Current commercial preservative systems maintain antimicrobial effectiveness for up to 8 weeks, suggesting guidelines for safe, planned replacement in clinical settings.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997
Externally publishedYes

Fingerprint

Benzalkonium Compounds
Ophthalmic Solutions
Tropicamide
Thimerosal
Bacteriology
Suspensions
Guidelines
Growth
polyaminopropylbiguanide
proxymetacaine

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Antibacterial effectiveness of commercial eyedrop preservatives. / Kirn, K. B.; Barequet, I.; Chan, T. K.; Osterhout, G. J.; O'Brien, Terrence.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 01.12.1997.

Research output: Contribution to journalArticle

Kirn, K. B. ; Barequet, I. ; Chan, T. K. ; Osterhout, G. J. ; O'Brien, Terrence. / Antibacterial effectiveness of commercial eyedrop preservatives. In: Investigative Ophthalmology and Visual Science. 1997 ; Vol. 38, No. 4.
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