TY - JOUR
T1 - Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice.
AU - Yo, Yoshikage
AU - Braun, Michael C.
AU - Barisoni, Laura
AU - Mobaraki, Hideko
AU - Lu, Huiyan
AU - Shrivastav, Shashi
AU - Owens, Jennie
AU - Kopp, Jeffrey B.
PY - 2003
Y1 - 2003
N2 - In order to develop a model in mouse similar to anti- Thy-1 nephritis in the rat, we prepared sheep antiserum against SV40-transformed mouse mesangial (MES 13) cells. In vivo, the anti-mouse mesangial cell serum-treated mice showed severe azotemia that peaked at day 6 and proteinuria that peaked at day 8, in a dose-dependent fashion. Light microscopy and electron microscopy showed duplication of glomerular basement membranes, mesangiolysis, subendothelial and mesangial electron-dense deposits, and foot process effacement. Intraglomerular tuft cell number was significantly reduced at day 4 and there were increased numbers of apoptotic cells at days 2 and 4. SCID mice and mice lacking C3 manifested similar responses to anti-mouse mesangial cell serum, suggesting that T cells, B cells and complement are not required for glomerular injury in this model. In vitro, anti-mouse mesangial cell serum treated mesangial cells showed greater release of lactate dehydrogenase, decreased cell survival, and increased apoptotic cell death. Anti-mouse mesangial cell serum induces glomerulopathy characterized by mesangiolysis and mesangial cell apoptosis, and followed by cellular proliferation.
AB - In order to develop a model in mouse similar to anti- Thy-1 nephritis in the rat, we prepared sheep antiserum against SV40-transformed mouse mesangial (MES 13) cells. In vivo, the anti-mouse mesangial cell serum-treated mice showed severe azotemia that peaked at day 6 and proteinuria that peaked at day 8, in a dose-dependent fashion. Light microscopy and electron microscopy showed duplication of glomerular basement membranes, mesangiolysis, subendothelial and mesangial electron-dense deposits, and foot process effacement. Intraglomerular tuft cell number was significantly reduced at day 4 and there were increased numbers of apoptotic cells at days 2 and 4. SCID mice and mice lacking C3 manifested similar responses to anti-mouse mesangial cell serum, suggesting that T cells, B cells and complement are not required for glomerular injury in this model. In vitro, anti-mouse mesangial cell serum treated mesangial cells showed greater release of lactate dehydrogenase, decreased cell survival, and increased apoptotic cell death. Anti-mouse mesangial cell serum induces glomerulopathy characterized by mesangiolysis and mesangial cell apoptosis, and followed by cellular proliferation.
UR - http://www.scopus.com/inward/record.url?scp=0037269428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037269428&partnerID=8YFLogxK
U2 - 10.1159/000069551
DO - 10.1159/000069551
M3 - Article
C2 - 12660412
AN - SCOPUS:0037269428
VL - 93
SP - e92-106
JO - Nephron - Experimental Nephrology
JF - Nephron - Experimental Nephrology
SN - 1660-2129
IS - 3
ER -