Anti-mouse CD122 antibody promotes the hematopoietic repopulating capacity of cord blood CD34⁺ cells in NOD/SCID mice

Men Yao Sheng, Hui Shi, Wen Xing, Wen Jun Wang, Xiao Hui Si, Jie Bai, Wei Ping Yuan, Yuan Zhou, Feng-Chun Yang

Research output: Contribution to journalArticle

Abstract

The study was aimed to investigate the effect of anti-mouse CD122 antibody on the hematopoietic repopulating capacity of cord blood CD34⁺ cells in a humanized murine model-non obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. After sublethal irradiation with γ-ray, NOD/SCID mice were intraperitoneally injected with 200 µg mouse isotype control antibody or anti-mouse CD122 antibody. Human cord blood CD34⁺ cells or phosphate-buffered saline (PBS) were injected via the tail vein at 6-8 hours later. Cohort of the mice injected with anti-mice CD122 antibody or control antibody alone were sacrificed at different time point (at week 2, 3, and 4 weeks) after the injection, and the percentage of NK cells in the peripheral blood was analyzed by flow cytometry. To evaluate the effect of anti-mouse CD122 antibody on the repopulating capacity of cord blood CD34⁺ cells in the recipient mice, phenotype analysis was performed in the bone marrow at 6 and 8 weeks after the transplantation. The results showed that the proportion of NK cells in the peripheral blood were (4.6 ± 0.6)% and (5.7 ± 1.7)% at week 2 and 3 after anti-CD122 antibody injection respectively,which decreased by 60%, compared with the mice injected with isotype control antibody. After 6 and 8 weeks of cord blood CD34⁺ cell transplantation,the percentage of human CD45⁺ in the bone marrow of the recipient mice treated with anti-mice CD122 antibody was (63.0 ± 12.2)% and (53.2 ± 16.3)%,respectively,which were dramatically higher than that in the mice treated with isotype control antibody (7.7 ± 3.6)% and (6.1 ± 2.4)%. Moreover,at 8 weeks after transplantation,human CD34⁺ cells appeared significantly in the recipients treated with anti-CD122 antibody. It is concluded that the anti-mouse CD122 antibody enhances the hematopoietic repopulating capacity of cord blood CD34⁺ cells in the NOD/SCID mice through decreasing the proportion of NK cells.

Original languageEnglish (US)
Pages (from-to)1673-1677
Number of pages5
JournalZhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology
Volume22
Issue number6
DOIs
StatePublished - Dec 1 2014
Externally publishedYes

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Severe Combined Immunodeficiency
Fetal Blood
Blood Cells
Antibodies
Natural Killer Cells
Anti-Idiotypic Antibodies
Transplantation
Bone Marrow
Injections
Cell Transplantation

ASJC Scopus subject areas

  • Medicine(all)

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Anti-mouse CD122 antibody promotes the hematopoietic repopulating capacity of cord blood CD34⁺ cells in NOD/SCID mice. / Sheng, Men Yao; Shi, Hui; Xing, Wen; Wang, Wen Jun; Si, Xiao Hui; Bai, Jie; Yuan, Wei Ping; Zhou, Yuan; Yang, Feng-Chun.

In: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 22, No. 6, 01.12.2014, p. 1673-1677.

Research output: Contribution to journalArticle

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abstract = "The study was aimed to investigate the effect of anti-mouse CD122 antibody on the hematopoietic repopulating capacity of cord blood CD34⁺ cells in a humanized murine model-non obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. After sublethal irradiation with γ-ray, NOD/SCID mice were intraperitoneally injected with 200 µg mouse isotype control antibody or anti-mouse CD122 antibody. Human cord blood CD34⁺ cells or phosphate-buffered saline (PBS) were injected via the tail vein at 6-8 hours later. Cohort of the mice injected with anti-mice CD122 antibody or control antibody alone were sacrificed at different time point (at week 2, 3, and 4 weeks) after the injection, and the percentage of NK cells in the peripheral blood was analyzed by flow cytometry. To evaluate the effect of anti-mouse CD122 antibody on the repopulating capacity of cord blood CD34⁺ cells in the recipient mice, phenotype analysis was performed in the bone marrow at 6 and 8 weeks after the transplantation. The results showed that the proportion of NK cells in the peripheral blood were (4.6 ± 0.6){\%} and (5.7 ± 1.7){\%} at week 2 and 3 after anti-CD122 antibody injection respectively,which decreased by 60{\%}, compared with the mice injected with isotype control antibody. After 6 and 8 weeks of cord blood CD34⁺ cell transplantation,the percentage of human CD45⁺ in the bone marrow of the recipient mice treated with anti-mice CD122 antibody was (63.0 ± 12.2){\%} and (53.2 ± 16.3){\%},respectively,which were dramatically higher than that in the mice treated with isotype control antibody (7.7 ± 3.6){\%} and (6.1 ± 2.4){\%}. Moreover,at 8 weeks after transplantation,human CD34⁺ cells appeared significantly in the recipients treated with anti-CD122 antibody. It is concluded that the anti-mouse CD122 antibody enhances the hematopoietic repopulating capacity of cord blood CD34⁺ cells in the NOD/SCID mice through decreasing the proportion of NK cells.",
author = "Sheng, {Men Yao} and Hui Shi and Wen Xing and Wang, {Wen Jun} and Si, {Xiao Hui} and Jie Bai and Yuan, {Wei Ping} and Yuan Zhou and Feng-Chun Yang",
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AU - Sheng, Men Yao

AU - Shi, Hui

AU - Xing, Wen

AU - Wang, Wen Jun

AU - Si, Xiao Hui

AU - Bai, Jie

AU - Yuan, Wei Ping

AU - Zhou, Yuan

AU - Yang, Feng-Chun

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