Anti-inflammatory activity of 5-O-demethylnobiletin, a polymethoxyflavone isolated from Sideritis tragoriganum

Esperanza Bas Infante, M. Carmen Recio, Rosa M. Giner, Salvador Máñez, Miguel Cerdá-Nicolás, José Luis Ríos

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have studied the effect of 5-O-demethylnobiletin (1) on both the inflammation of mouse ears induced by repeated application of 12-O-tetradecanoylphorbol 13-acetate (TPA) and the acute mouse paw oedemas induced by carrageenan and phospholipase A2 (PLA2), and determined its activity on 5-lipoxygenase (5-LOX) and elastase release/activity. Compound 1 reduced the oedema formation, cell infiltration, and tissue damage in the inflammation induced by TPA in mouse ears, along with the acute oedema induced by carrageenan in mouse paws and the acute PLA2-induced oedema in mouse paws. The flavone inhibited leukotriene B4 formation in rat neutrophils and elastase release in human neutrophils, but did not reduce the expression of cyclooxygenase-2 (COX-2) in murine RAW 264.7 macrophages. These experimental results suggest that 1 may act through a direct inhibition of 5-LOX, without affecting the expression of COX-2.

Original languageEnglish (US)
Pages (from-to)136-142
Number of pages7
JournalPlanta Medica
Volume72
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Sideritis
Arachidonate 5-Lipoxygenase
flavone
Carrageenan
Phospholipases A2
Cyclooxygenase 2
anti-inflammatory activity
Acetates
Anti-Inflammatory Agents
Edema
edema
Leukocyte Elastase
Leukotriene B4
Macrophages
Pancreatic Elastase
mice
Infiltration
Rats
Tetradecanoylphorbol Acetate
elastase

Keywords

  • 5-lipoxygenase
  • 5-O-Demethylnobiletin
  • Anti-inflammatory activity
  • Lamiaceae
  • Sideritis tragoriganum

ASJC Scopus subject areas

  • Plant Science
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Anti-inflammatory activity of 5-O-demethylnobiletin, a polymethoxyflavone isolated from Sideritis tragoriganum. / Bas Infante, Esperanza; Recio, M. Carmen; Giner, Rosa M.; Máñez, Salvador; Cerdá-Nicolás, Miguel; Ríos, José Luis.

In: Planta Medica, Vol. 72, No. 2, 02.2006, p. 136-142.

Research output: Contribution to journalArticle

Bas Infante, Esperanza ; Recio, M. Carmen ; Giner, Rosa M. ; Máñez, Salvador ; Cerdá-Nicolás, Miguel ; Ríos, José Luis. / Anti-inflammatory activity of 5-O-demethylnobiletin, a polymethoxyflavone isolated from Sideritis tragoriganum. In: Planta Medica. 2006 ; Vol. 72, No. 2. pp. 136-142.
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