Anti-citrullinated heat shock protein 90 antibodies identified in bronchoalveolar lavage fluid are a marker of lung-specific immune responses

Lisa Harlow, Bernadette R. Gochuico, Ivan O. Rosas, Tracy J. Doyle, Juan C. Osorio, Timothy S. Travers, Carlos C. Camacho, Chester V. Oddis, Dana Ascherman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Previous work has demonstrated a correlation between serum anti-citrullinated HSP90 antibodies and rheumatoid arthritis-associated interstitial lung disease (RA-ILD). To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. 9/21 RA-derived BALF specimens demonstrated IgG and/or IgA antibodies targeting citrullinated HSP90 proteins/peptides, highlighting disease specific responses (with a predilection for RA-ILD) that did not occur in IPF patients (0/5) or healthy control subjects (0/5). Comparison of antibody profiles between BALF and matching serum specimens revealed various recognition patterns favoring predominant production of anti-citrullinated HSP90 antibodies within the lung microenvironment-further supporting the connection between this antibody specificity and parenchymal lung disease. Equally important, qualitative as well as quantitative differences in anti-citrullinated HSP90 profiles between BALF and serum indicate that the lung plays a direct role in shaping the immune repertoire of RA/RA-ILD.

Original languageEnglish
Pages (from-to)60-70
Number of pages11
JournalClinical Immunology
Volume155
Issue number1
DOIs
StatePublished - Aug 11 2014

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HSP90 Heat-Shock Proteins
Bronchoalveolar Lavage Fluid
Interstitial Lung Diseases
Rheumatoid Arthritis
Lung
Antibodies
Serum
Antibody Specificity
Immunoglobulin A
Lung Diseases
Healthy Volunteers
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Peptides
Proteins

Keywords

  • Autoantibodies
  • Bronchoalveolar lavage fluid
  • Citrullination
  • HSP90
  • Interstitial lung disease
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Anti-citrullinated heat shock protein 90 antibodies identified in bronchoalveolar lavage fluid are a marker of lung-specific immune responses. / Harlow, Lisa; Gochuico, Bernadette R.; Rosas, Ivan O.; Doyle, Tracy J.; Osorio, Juan C.; Travers, Timothy S.; Camacho, Carlos C.; Oddis, Chester V.; Ascherman, Dana.

In: Clinical Immunology, Vol. 155, No. 1, 11.08.2014, p. 60-70.

Research output: Contribution to journalArticle

Harlow, Lisa ; Gochuico, Bernadette R. ; Rosas, Ivan O. ; Doyle, Tracy J. ; Osorio, Juan C. ; Travers, Timothy S. ; Camacho, Carlos C. ; Oddis, Chester V. ; Ascherman, Dana. / Anti-citrullinated heat shock protein 90 antibodies identified in bronchoalveolar lavage fluid are a marker of lung-specific immune responses. In: Clinical Immunology. 2014 ; Vol. 155, No. 1. pp. 60-70.
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