Anti-citrullinated heat shock protein 90 antibodies identified in bronchoalveolar lavage fluid are a marker of lung-specific immune responses

Lisa Harlow, Bernadette R. Gochuico, Ivan O. Rosas, Tracy J. Doyle, Juan C. Osorio, Timothy S. Travers, Carlos C. Camacho, Chester V. Oddis, Dana Ascherman

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14 Scopus citations

Abstract

Previous work has demonstrated a correlation between serum anti-citrullinated HSP90 antibodies and rheumatoid arthritis-associated interstitial lung disease (RA-ILD). To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. 9/21 RA-derived BALF specimens demonstrated IgG and/or IgA antibodies targeting citrullinated HSP90 proteins/peptides, highlighting disease specific responses (with a predilection for RA-ILD) that did not occur in IPF patients (0/5) or healthy control subjects (0/5). Comparison of antibody profiles between BALF and matching serum specimens revealed various recognition patterns favoring predominant production of anti-citrullinated HSP90 antibodies within the lung microenvironment-further supporting the connection between this antibody specificity and parenchymal lung disease. Equally important, qualitative as well as quantitative differences in anti-citrullinated HSP90 profiles between BALF and serum indicate that the lung plays a direct role in shaping the immune repertoire of RA/RA-ILD.

Original languageEnglish
Pages (from-to)60-70
Number of pages11
JournalClinical Immunology
Volume155
Issue number1
DOIs
StatePublished - Aug 11 2014

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Keywords

  • Autoantibodies
  • Bronchoalveolar lavage fluid
  • Citrullination
  • HSP90
  • Interstitial lung disease
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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