Anti-citrullinated heat shock protein 90 antibodies identified in bronchoalveolar lavage fluid are a marker of lung-specific immune responses

Lisa Harlow, Bernadette R. Gochuico, Ivan O. Rosas, Tracy J. Doyle, Juan C. Osorio, Timothy S. Travers, Carlos C. Camacho, Chester V. Oddis, Dana P. Ascherman

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Previous work has demonstrated a correlation between serum anti-citrullinated HSP90 antibodies and rheumatoid arthritis-associated interstitial lung disease (RA-ILD). To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. 9/21 RA-derived BALF specimens demonstrated IgG and/or IgA antibodies targeting citrullinated HSP90 proteins/peptides, highlighting disease specific responses (with a predilection for RA-ILD) that did not occur in IPF patients (0/5) or healthy control subjects (0/5). Comparison of antibody profiles between BALF and matching serum specimens revealed various recognition patterns favoring predominant production of anti-citrullinated HSP90 antibodies within the lung microenvironment-further supporting the connection between this antibody specificity and parenchymal lung disease. Equally important, qualitative as well as quantitative differences in anti-citrullinated HSP90 profiles between BALF and serum indicate that the lung plays a direct role in shaping the immune repertoire of RA/RA-ILD.

Original languageEnglish (US)
Pages (from-to)60-70
Number of pages11
JournalClinical Immunology
Volume155
Issue number1
DOIs
StatePublished - Aug 11 2014

Keywords

  • Autoantibodies
  • Bronchoalveolar lavage fluid
  • Citrullination
  • HSP90
  • Interstitial lung disease
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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