Abstract
Previous work has demonstrated a correlation between serum anti-citrullinated HSP90 antibodies and rheumatoid arthritis-associated interstitial lung disease (RA-ILD). To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. 9/21 RA-derived BALF specimens demonstrated IgG and/or IgA antibodies targeting citrullinated HSP90 proteins/peptides, highlighting disease specific responses (with a predilection for RA-ILD) that did not occur in IPF patients (0/5) or healthy control subjects (0/5). Comparison of antibody profiles between BALF and matching serum specimens revealed various recognition patterns favoring predominant production of anti-citrullinated HSP90 antibodies within the lung microenvironment-further supporting the connection between this antibody specificity and parenchymal lung disease. Equally important, qualitative as well as quantitative differences in anti-citrullinated HSP90 profiles between BALF and serum indicate that the lung plays a direct role in shaping the immune repertoire of RA/RA-ILD.
| Original language | English |
|---|---|
| Pages (from-to) | 60-70 |
| Number of pages | 11 |
| Journal | Clinical Immunology |
| Volume | 155 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 11 2014 |
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Keywords
- Autoantibodies
- Bronchoalveolar lavage fluid
- Citrullination
- HSP90
- Interstitial lung disease
- Rheumatoid arthritis
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Anti-citrullinated heat shock protein 90 antibodies identified in bronchoalveolar lavage fluid are a marker of lung-specific immune responses. / Harlow, Lisa; Gochuico, Bernadette R.; Rosas, Ivan O.; Doyle, Tracy J.; Osorio, Juan C.; Travers, Timothy S.; Camacho, Carlos C.; Oddis, Chester V.; Ascherman, Dana.
In: Clinical Immunology, Vol. 155, No. 1, 11.08.2014, p. 60-70.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-citrullinated heat shock protein 90 antibodies identified in bronchoalveolar lavage fluid are a marker of lung-specific immune responses
AU - Harlow, Lisa
AU - Gochuico, Bernadette R.
AU - Rosas, Ivan O.
AU - Doyle, Tracy J.
AU - Osorio, Juan C.
AU - Travers, Timothy S.
AU - Camacho, Carlos C.
AU - Oddis, Chester V.
AU - Ascherman, Dana
PY - 2014/8/11
Y1 - 2014/8/11
N2 - Previous work has demonstrated a correlation between serum anti-citrullinated HSP90 antibodies and rheumatoid arthritis-associated interstitial lung disease (RA-ILD). To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. 9/21 RA-derived BALF specimens demonstrated IgG and/or IgA antibodies targeting citrullinated HSP90 proteins/peptides, highlighting disease specific responses (with a predilection for RA-ILD) that did not occur in IPF patients (0/5) or healthy control subjects (0/5). Comparison of antibody profiles between BALF and matching serum specimens revealed various recognition patterns favoring predominant production of anti-citrullinated HSP90 antibodies within the lung microenvironment-further supporting the connection between this antibody specificity and parenchymal lung disease. Equally important, qualitative as well as quantitative differences in anti-citrullinated HSP90 profiles between BALF and serum indicate that the lung plays a direct role in shaping the immune repertoire of RA/RA-ILD.
AB - Previous work has demonstrated a correlation between serum anti-citrullinated HSP90 antibodies and rheumatoid arthritis-associated interstitial lung disease (RA-ILD). To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. 9/21 RA-derived BALF specimens demonstrated IgG and/or IgA antibodies targeting citrullinated HSP90 proteins/peptides, highlighting disease specific responses (with a predilection for RA-ILD) that did not occur in IPF patients (0/5) or healthy control subjects (0/5). Comparison of antibody profiles between BALF and matching serum specimens revealed various recognition patterns favoring predominant production of anti-citrullinated HSP90 antibodies within the lung microenvironment-further supporting the connection between this antibody specificity and parenchymal lung disease. Equally important, qualitative as well as quantitative differences in anti-citrullinated HSP90 profiles between BALF and serum indicate that the lung plays a direct role in shaping the immune repertoire of RA/RA-ILD.
KW - Autoantibodies
KW - Bronchoalveolar lavage fluid
KW - Citrullination
KW - HSP90
KW - Interstitial lung disease
KW - Rheumatoid arthritis
UR - http://www.scopus.com/inward/record.url?scp=84907176207&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907176207&partnerID=8YFLogxK
U2 - 10.1016/j.clim.2014.08.004
DO - 10.1016/j.clim.2014.08.004
M3 - Article
C2 - 25150019
AN - SCOPUS:84907176207
VL - 155
SP - 60
EP - 70
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 1
ER -