Anti-ceramidase LCL385 acutely reduces BCL-2 expression in the hippocampus but is not associated with an increase of learned helplessness in rats

Ziad Nahas, Yan Jiang, Youssef Zeidan, Alicja Bielawska, Zdzislaw Szulc, Lindsay DeVane, Peter Kalivas, Yusuf A. Hannun

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Evidence from in situ studies supports the role of anti-apoptotic factors in the antidepressant responses of certain psychotropics. The availability of anti-ceramidase pro-apoptocic compound (LCL385) provides an opportunity to test in vivo the relation between hippocampal apopotosis and learned helplessness. Method: 40 Sprague-Dawley male rodents underwent an FST after a treatment with LCL385, desipramine (DMI), or placebo (SAL) over 3 days. Behavioral responses, including immobility, swimming and climbing were counted during the 6 min test. Western blot labeling was used to detect anti-apoptosis in hippocampus. Results: DMI alone was associated with reduced immobility and increased climbing whereas LCL385 alone showed a decrease in Bcl-2/β-actin ratio. Conclusions: Direct modulation of Bcl-2 expression in the hippocampus is not associated with learned helplessness in stressed rats. Three-day administration of DMI and LCL385 show divergent effects on behavioral and anti-apoptotic measures.

Original languageEnglish (US)
Pages (from-to)41-44
Number of pages4
JournalBehavioural Brain Research
Volume197
Issue number1
DOIs
StatePublished - Jan 30 2009
Externally publishedYes

Fingerprint

Ceramidases
Learned Helplessness
Desipramine
Hippocampus
Antidepressive Agents
Actins
Rodentia
Western Blotting
Placebos
Apoptosis
2-N-(tetradecylamino)-1-phenyl-1,3-propanediol

Keywords

  • Apoptosis
  • Bcl-2
  • Ceramide
  • Depression
  • Despair
  • FST
  • Helplessness
  • Hippocampus
  • Rodent
  • Stress

ASJC Scopus subject areas

  • Behavioral Neuroscience

Cite this

Anti-ceramidase LCL385 acutely reduces BCL-2 expression in the hippocampus but is not associated with an increase of learned helplessness in rats. / Nahas, Ziad; Jiang, Yan; Zeidan, Youssef; Bielawska, Alicja; Szulc, Zdzislaw; DeVane, Lindsay; Kalivas, Peter; Hannun, Yusuf A.

In: Behavioural Brain Research, Vol. 197, No. 1, 30.01.2009, p. 41-44.

Research output: Contribution to journalArticle

Nahas, Ziad ; Jiang, Yan ; Zeidan, Youssef ; Bielawska, Alicja ; Szulc, Zdzislaw ; DeVane, Lindsay ; Kalivas, Peter ; Hannun, Yusuf A. / Anti-ceramidase LCL385 acutely reduces BCL-2 expression in the hippocampus but is not associated with an increase of learned helplessness in rats. In: Behavioural Brain Research. 2009 ; Vol. 197, No. 1. pp. 41-44.
@article{bdc1429641e6425691760ddbcb62503a,
title = "Anti-ceramidase LCL385 acutely reduces BCL-2 expression in the hippocampus but is not associated with an increase of learned helplessness in rats",
abstract = "Evidence from in situ studies supports the role of anti-apoptotic factors in the antidepressant responses of certain psychotropics. The availability of anti-ceramidase pro-apoptocic compound (LCL385) provides an opportunity to test in vivo the relation between hippocampal apopotosis and learned helplessness. Method: 40 Sprague-Dawley male rodents underwent an FST after a treatment with LCL385, desipramine (DMI), or placebo (SAL) over 3 days. Behavioral responses, including immobility, swimming and climbing were counted during the 6 min test. Western blot labeling was used to detect anti-apoptosis in hippocampus. Results: DMI alone was associated with reduced immobility and increased climbing whereas LCL385 alone showed a decrease in Bcl-2/β-actin ratio. Conclusions: Direct modulation of Bcl-2 expression in the hippocampus is not associated with learned helplessness in stressed rats. Three-day administration of DMI and LCL385 show divergent effects on behavioral and anti-apoptotic measures.",
keywords = "Apoptosis, Bcl-2, Ceramide, Depression, Despair, FST, Helplessness, Hippocampus, Rodent, Stress",
author = "Ziad Nahas and Yan Jiang and Youssef Zeidan and Alicja Bielawska and Zdzislaw Szulc and Lindsay DeVane and Peter Kalivas and Hannun, {Yusuf A.}",
year = "2009",
month = "1",
day = "30",
doi = "10.1016/j.bbr.2008.07.040",
language = "English (US)",
volume = "197",
pages = "41--44",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Anti-ceramidase LCL385 acutely reduces BCL-2 expression in the hippocampus but is not associated with an increase of learned helplessness in rats

AU - Nahas, Ziad

AU - Jiang, Yan

AU - Zeidan, Youssef

AU - Bielawska, Alicja

AU - Szulc, Zdzislaw

AU - DeVane, Lindsay

AU - Kalivas, Peter

AU - Hannun, Yusuf A.

PY - 2009/1/30

Y1 - 2009/1/30

N2 - Evidence from in situ studies supports the role of anti-apoptotic factors in the antidepressant responses of certain psychotropics. The availability of anti-ceramidase pro-apoptocic compound (LCL385) provides an opportunity to test in vivo the relation between hippocampal apopotosis and learned helplessness. Method: 40 Sprague-Dawley male rodents underwent an FST after a treatment with LCL385, desipramine (DMI), or placebo (SAL) over 3 days. Behavioral responses, including immobility, swimming and climbing were counted during the 6 min test. Western blot labeling was used to detect anti-apoptosis in hippocampus. Results: DMI alone was associated with reduced immobility and increased climbing whereas LCL385 alone showed a decrease in Bcl-2/β-actin ratio. Conclusions: Direct modulation of Bcl-2 expression in the hippocampus is not associated with learned helplessness in stressed rats. Three-day administration of DMI and LCL385 show divergent effects on behavioral and anti-apoptotic measures.

AB - Evidence from in situ studies supports the role of anti-apoptotic factors in the antidepressant responses of certain psychotropics. The availability of anti-ceramidase pro-apoptocic compound (LCL385) provides an opportunity to test in vivo the relation between hippocampal apopotosis and learned helplessness. Method: 40 Sprague-Dawley male rodents underwent an FST after a treatment with LCL385, desipramine (DMI), or placebo (SAL) over 3 days. Behavioral responses, including immobility, swimming and climbing were counted during the 6 min test. Western blot labeling was used to detect anti-apoptosis in hippocampus. Results: DMI alone was associated with reduced immobility and increased climbing whereas LCL385 alone showed a decrease in Bcl-2/β-actin ratio. Conclusions: Direct modulation of Bcl-2 expression in the hippocampus is not associated with learned helplessness in stressed rats. Three-day administration of DMI and LCL385 show divergent effects on behavioral and anti-apoptotic measures.

KW - Apoptosis

KW - Bcl-2

KW - Ceramide

KW - Depression

KW - Despair

KW - FST

KW - Helplessness

KW - Hippocampus

KW - Rodent

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=57649234857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57649234857&partnerID=8YFLogxK

U2 - 10.1016/j.bbr.2008.07.040

DO - 10.1016/j.bbr.2008.07.040

M3 - Article

VL - 197

SP - 41

EP - 44

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

IS - 1

ER -