Anti-CD36 autoantibodies in thrombotic thrombocytopenic purpura and other thrombotic disorders

Identification of an 85 kD form of CD36 as a target antigen

Duane R. Schultz, Patricia I. Arnold, Wenche Jy, Peter A. Valant, Julie Gruber, Yeon Ahn, Fang W. Mao, Wei W. Mao, Larry L. Horstman

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The presence of anti-CD36 antibodies in plasma of patients with thrombotic thrombocytopenic purpura (TTP), idiopathic thrombocytopenic purpura (ITP), and heparin-induced thrombocytopenia without/with thrombosis (HIT/HITT) has been examined by immunoblots, and a monoclonal antibody capture assay, the platelet-associated IgG characterization assay (PAICA). Results with PAICA showed that 73% (8/11) of patients with TTP were positive, and 71% (10/14) by immunoblots. With ITP, 20% (6/30) were positive by PAICA and 19% (3/16) by immunoblots; HIT, 30% (3/10) were positive by PAICA and 60% (6/10) by immunoblot; HITT, 50% (2/4) by PAICA and 100% (4/4) by immunoblot. Purification of CD36 by fast protein liquid chromatography (FPLC) from Triton X-100 extracts of normal platelet membranes resulted in the isolation of two different forms: the classic 88 kD form, and a second, lighter 85 kD form. Our data indicated that the patients' plasma autoantibodies reacted strongly with the 85 kD form. Conventional monoclonal and polyclonal antisera produced to the 88 kD form reacted strongly with the 88 kD form but weakly with the 85 kD form. These results confirm the possible importance of anti-CD36 antibodies in the pathophysiology of TTP and other thrombocytopenias and demonstrate the presence of a previously unrecognized target antigen for these antibodies.

Original languageEnglish
Pages (from-to)849-857
Number of pages9
JournalBritish Journal of Haematology
Volume103
Issue number3
DOIs
StatePublished - Dec 10 1998

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Thrombotic Thrombocytopenic Purpura
Autoantibodies
Blood Platelets
Antigens
Immunoglobulin G
Idiopathic Thrombocytopenic Purpura
Anti-Idiotypic Antibodies
CD36 Antigens
Octoxynol
Liquid Chromatography
Thrombocytopenia
Heparin
Immune Sera
Thrombosis
Monoclonal Antibodies
Membranes
Antibodies

Keywords

  • Glycoform
  • Immunoassays
  • Platelets
  • Thrombocytopenia
  • Thrombosis

ASJC Scopus subject areas

  • Hematology

Cite this

Anti-CD36 autoantibodies in thrombotic thrombocytopenic purpura and other thrombotic disorders : Identification of an 85 kD form of CD36 as a target antigen. / Schultz, Duane R.; Arnold, Patricia I.; Jy, Wenche; Valant, Peter A.; Gruber, Julie; Ahn, Yeon; Mao, Fang W.; Mao, Wei W.; Horstman, Larry L.

In: British Journal of Haematology, Vol. 103, No. 3, 10.12.1998, p. 849-857.

Research output: Contribution to journalArticle

Schultz, Duane R. ; Arnold, Patricia I. ; Jy, Wenche ; Valant, Peter A. ; Gruber, Julie ; Ahn, Yeon ; Mao, Fang W. ; Mao, Wei W. ; Horstman, Larry L. / Anti-CD36 autoantibodies in thrombotic thrombocytopenic purpura and other thrombotic disorders : Identification of an 85 kD form of CD36 as a target antigen. In: British Journal of Haematology. 1998 ; Vol. 103, No. 3. pp. 849-857.
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abstract = "The presence of anti-CD36 antibodies in plasma of patients with thrombotic thrombocytopenic purpura (TTP), idiopathic thrombocytopenic purpura (ITP), and heparin-induced thrombocytopenia without/with thrombosis (HIT/HITT) has been examined by immunoblots, and a monoclonal antibody capture assay, the platelet-associated IgG characterization assay (PAICA). Results with PAICA showed that 73{\%} (8/11) of patients with TTP were positive, and 71{\%} (10/14) by immunoblots. With ITP, 20{\%} (6/30) were positive by PAICA and 19{\%} (3/16) by immunoblots; HIT, 30{\%} (3/10) were positive by PAICA and 60{\%} (6/10) by immunoblot; HITT, 50{\%} (2/4) by PAICA and 100{\%} (4/4) by immunoblot. Purification of CD36 by fast protein liquid chromatography (FPLC) from Triton X-100 extracts of normal platelet membranes resulted in the isolation of two different forms: the classic 88 kD form, and a second, lighter 85 kD form. Our data indicated that the patients' plasma autoantibodies reacted strongly with the 85 kD form. Conventional monoclonal and polyclonal antisera produced to the 88 kD form reacted strongly with the 88 kD form but weakly with the 85 kD form. These results confirm the possible importance of anti-CD36 antibodies in the pathophysiology of TTP and other thrombocytopenias and demonstrate the presence of a previously unrecognized target antigen for these antibodies.",
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T2 - Identification of an 85 kD form of CD36 as a target antigen

AU - Schultz, Duane R.

AU - Arnold, Patricia I.

AU - Jy, Wenche

AU - Valant, Peter A.

AU - Gruber, Julie

AU - Ahn, Yeon

AU - Mao, Fang W.

AU - Mao, Wei W.

AU - Horstman, Larry L.

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N2 - The presence of anti-CD36 antibodies in plasma of patients with thrombotic thrombocytopenic purpura (TTP), idiopathic thrombocytopenic purpura (ITP), and heparin-induced thrombocytopenia without/with thrombosis (HIT/HITT) has been examined by immunoblots, and a monoclonal antibody capture assay, the platelet-associated IgG characterization assay (PAICA). Results with PAICA showed that 73% (8/11) of patients with TTP were positive, and 71% (10/14) by immunoblots. With ITP, 20% (6/30) were positive by PAICA and 19% (3/16) by immunoblots; HIT, 30% (3/10) were positive by PAICA and 60% (6/10) by immunoblot; HITT, 50% (2/4) by PAICA and 100% (4/4) by immunoblot. Purification of CD36 by fast protein liquid chromatography (FPLC) from Triton X-100 extracts of normal platelet membranes resulted in the isolation of two different forms: the classic 88 kD form, and a second, lighter 85 kD form. Our data indicated that the patients' plasma autoantibodies reacted strongly with the 85 kD form. Conventional monoclonal and polyclonal antisera produced to the 88 kD form reacted strongly with the 88 kD form but weakly with the 85 kD form. These results confirm the possible importance of anti-CD36 antibodies in the pathophysiology of TTP and other thrombocytopenias and demonstrate the presence of a previously unrecognized target antigen for these antibodies.

AB - The presence of anti-CD36 antibodies in plasma of patients with thrombotic thrombocytopenic purpura (TTP), idiopathic thrombocytopenic purpura (ITP), and heparin-induced thrombocytopenia without/with thrombosis (HIT/HITT) has been examined by immunoblots, and a monoclonal antibody capture assay, the platelet-associated IgG characterization assay (PAICA). Results with PAICA showed that 73% (8/11) of patients with TTP were positive, and 71% (10/14) by immunoblots. With ITP, 20% (6/30) were positive by PAICA and 19% (3/16) by immunoblots; HIT, 30% (3/10) were positive by PAICA and 60% (6/10) by immunoblot; HITT, 50% (2/4) by PAICA and 100% (4/4) by immunoblot. Purification of CD36 by fast protein liquid chromatography (FPLC) from Triton X-100 extracts of normal platelet membranes resulted in the isolation of two different forms: the classic 88 kD form, and a second, lighter 85 kD form. Our data indicated that the patients' plasma autoantibodies reacted strongly with the 85 kD form. Conventional monoclonal and polyclonal antisera produced to the 88 kD form reacted strongly with the 88 kD form but weakly with the 85 kD form. These results confirm the possible importance of anti-CD36 antibodies in the pathophysiology of TTP and other thrombocytopenias and demonstrate the presence of a previously unrecognized target antigen for these antibodies.

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KW - Immunoassays

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