Anoxic survival of the isolated cerebellum of the turtle Pseudemis scripta elegans

Miguel A. Pérez-Pinzón, Myron Rosenthal, Peter L. Lutz, Thomas J. Sick

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Intact turtle brain provides a useful model for the study of anoxia and potential survival strategies, since this tissue maintains transmembrane ion gradients and ATP levels during prolonged anoxia and recovers functional activity afterwards. Since isolated tissues offer experimental advantages, the present study sought to determine effects of anoxia on the isolated turtle cerebellum and to define relationships between anoxia survival and glucose supply. In normoxia, the extracellular potassium ([K+]0) activity and evoked potentials were maintained with 5 m M glucose, but 20 m M glucose was required to maintain adenosine triphosphate (ATP) levels and prevent significant increases in [K+]0 during anoxia. Inhibition of glycolysis by iodoacetic acid (IAA) during anoxia provoked large increases in [K+]0 at all glucose levels. These results demonstrate the usefulness of the isolated turtle cerebellum for studies of anoxic survival since this tissue can maintain ATP levels and [K+]0 during prolonged anoxia with 20 m M glucose in the artificial cerebrospinal fluid medium. They also suggest the presence of a Pasteur effect at least during the transition to a hypometabolic state.

Original languageEnglish (US)
Pages (from-to)68-73
Number of pages6
JournalJournal of Comparative Physiology B
Volume162
Issue number1
DOIs
StatePublished - Jan 1 1992

Keywords

  • Anoxia
  • Cerebellum
  • Glucose
  • Potassium
  • Turtle, Pseudemis

ASJC Scopus subject areas

  • Physiology (medical)
  • Environmental Science(all)
  • Physiology
  • Animal Science and Zoology

Fingerprint Dive into the research topics of 'Anoxic survival of the isolated cerebellum of the turtle Pseudemis scripta elegans'. Together they form a unique fingerprint.

  • Cite this