Anoxia, acidosis, and intergenic interactions selectively regulate methionine sulfoxide reductase transcriptions in mouse embryonic stem cells

Chi Zhang, Pingping Jia, Yuanyuan Jia, Yuejin Li, Keith A Webster, Xupei Huang, Mohan Achary, Sharon L. Lemanski, Larry F. Lemanski

Research output: Contribution to journalArticle

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Abstract

Methionine sulfoxide reductases (Msr) belong to a gene family that contains one MsrA and three MsrBs (MsrB1, MsrB2, and MsrB3). We have identified all four of the genes that are expressed in mouse embryonic stem cell cultures. The vital cellular functions of the Msr family of genes are to protect cells from oxidative damage by enzymatically reducing the oxidized sulfide groups of methionine residues in proteins from the sulfoxide form (-SO) back to sulfide thus restoring normal protein functions as well as reducing intracellular reactive oxygen species (ROS). We have performed studies on the Msr family genes to examine the regulation of gene expression. Our studies using real-time RT-PCR and Western blotting have shown that expression levels of the four Msr family genes are under differential regulation by anoxia/reoxygenation treatment, acidic culture conditions and interactions between MsrA and MsrB. Results from these in vitro experiments suggest that although these genes function as a whole in oxidative stress protection, each one of the Msr genes could be responsive to environmental stimulants differently at the tissue level.

Original languageEnglish
Pages (from-to)98-106
Number of pages9
JournalJournal of Cellular Biochemistry
Volume112
Issue number1
DOIs
StatePublished - Jan 1 2011

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Methionine Sulfoxide Reductases
Transcription
Acidosis
Stem cells
Genes
sulfoxide
Sulfides
Oxidative stress
Gene Expression Regulation
methionine sulfoxide reductase
Mouse Embryonic Stem Cells
Hypoxia
Cell culture
Gene expression
Methionine
Real-Time Polymerase Chain Reaction
Reactive Oxygen Species
Proteins
Oxidative Stress
Cell Culture Techniques

Keywords

  • Acidosis
  • Anoxia
  • Mouse stem cells
  • Msr gene regulation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Anoxia, acidosis, and intergenic interactions selectively regulate methionine sulfoxide reductase transcriptions in mouse embryonic stem cells. / Zhang, Chi; Jia, Pingping; Jia, Yuanyuan; Li, Yuejin; Webster, Keith A; Huang, Xupei; Achary, Mohan; Lemanski, Sharon L.; Lemanski, Larry F.

In: Journal of Cellular Biochemistry, Vol. 112, No. 1, 01.01.2011, p. 98-106.

Research output: Contribution to journalArticle

Zhang, Chi ; Jia, Pingping ; Jia, Yuanyuan ; Li, Yuejin ; Webster, Keith A ; Huang, Xupei ; Achary, Mohan ; Lemanski, Sharon L. ; Lemanski, Larry F. / Anoxia, acidosis, and intergenic interactions selectively regulate methionine sulfoxide reductase transcriptions in mouse embryonic stem cells. In: Journal of Cellular Biochemistry. 2011 ; Vol. 112, No. 1. pp. 98-106.
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